CoVFlu One Step RT PCR Kit

Multiplex Detection of COVID-19, Influenza A/B & RSV by Real-Time RT-PCR

The CoVFlu One Step RT PCR Kit is a CE-IVD molecular diagnostic assay designed for the qualitative detection of SARS-CoV-2 (COVID-19), Influenza A, Influenza B, and Respiratory Syncytial Virus (RSV) in clinical samples.

This multiplex assay supports rapid differential diagnosis of viral respiratory infections, enabling timely clinical decisions, infection control, and outbreak surveillance through a single real-time RT-PCR workflow.

Product Overview

The CoVFlu One Step RT PCR Kit is an in vitro diagnostic (IVD) test based on advanced real-time RT-PCR technology for simultaneous detection of multiple respiratory viruses from a single patient sample.

The assay uses a single-tube multiplex design integrating Viral RNA extraction, Reverse transcription and Real-time PCR amplification within one streamlined workflow.

Why Multiplex Respiratory Testing Matters

Respiratory viral infections often present with overlapping symptoms, including:

Differentiating between COVID-19, Influenza, and RSV is clinically critical because:

Multiplex RT-PCR enables rapid pathogen identification in a single test, improving patient management and infection prevention.

Pathogens Detected

Viral Targets
  • SARS-CoV-2 (COVID-19)
  • Influenza A/B
  • Respiratory Syncytial Virus (RSV)
Internal Control
  • Human RNaseP gene
    Ensures RNA extraction quality and assay validity.

Applications

The kit is ideal for clinical molecular laboratories performing:

BCR-ABL Universal
  • COVID-19 RT-PCR testing
  • Influenza A/B detection
  • RSV detection
  • Multiplex respiratory pathogen screening
  • Differential diagnosis of viral respiratory infections
  • Outbreak surveillance and infection control programs

Key Features & Benefits

4-Pathogen Multiplex Detection

Simultaneous identification of COVID-19, Flu A/B, and RSV in one assay.

Single-Tube Real-Time RT-PCR Design

Simplifies workflow and reduces reagent handling.

Rapid Differential Diagnosis

Supports faster clinical triaging and treatment decisions.

Optimized for Syndromic Respiratory Testing

Ideal for seasonal flu, pandemic surveillance, and hospital screening.

Sample Types & Reporting Output

Sample Types

Validated for use with respiratory specimens, including:

  • Nasopharyngeal swabs
  • Oropharyngeal swabs
  • (Samples should be transported and stored at 2–8°C and processed within recommended timelines.)
Reporting Output

The assay provides clear qualitative reporting, including:

  • Pathogen-specific Positive / Negative result
  • Ct values for each target
  • Amplification curves
  • Internal control validation
  • Exportable reports via USB / Bluetooth (system dependent)

Compatible System

OnePCR-Rapi-Q Ultra-Fast POC System

Designed for automated workflow including extraction and amplification.

OnePCR® workflow
  • Collect NP/OP swab sample
  • Load 200 µl sample into Cartridge-1
  • Reconstitute lyophilized Ready Mix (Cartridge-2)
  • Insert cartridges into system
  • Automated RNA extraction + multiplex RT-PCR
  • Results displayed as pathogen-specific Positive/Negative with Ct curves

Storage & Stability

Ordering Information

Commercial Name Old Cat No. New Cat No. Pack Size
CoVFlu One Step RT PCR Kit G2M272721R 24 Tests

Why Choose the CoVFlu One Step RT PCR Kit?

If your lab requires a reliable multiplex respiratory RT-PCR kit, this assay delivers:

  • simultaneous detection of COVID-19, Flu A/B, and RSV
  • syndromic respiratory testing in a single assay
  • multiplex probe-based PCR chemistry
  • internal control-validated results
  • automated extraction-to-result workflow
  • suitability for hospital, reference lab, and surveillance testing

Resources to get started

Download useful documents and technical information for the CoVFlu One Step RT PCR Kit .

Product Brochure
IFU (Instructions for Use)
Report File

Product Enquiry Form

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Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.

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