OncoCheck NGS Panel

Overview


OncoCheck NGS assay is aimed to screen a range of disease causing genes to identify somatic mutations and germline mutations in DNA from FFPE, fresh tissue, and blood targeting 53 genes covering all the coding sequences enriched by hybridization capture-based target enrichment methodology. Genes are selected based on AMP/ASCO/CAP guidelines to uncover the coding region compiling to the size of 0.17 Mb.

Key features & highlights


  • Comprehensive Gene Coverage: Targets 53 clinically relevant genes associated with somatic and germline mutations, ensuring broad disease screening.
  • Full Coding Region Analysis: Covers entire coding sequences (0.17 Mb) for accurate mutation detection and interpretation.
  • Advanced Enrichment Technology: Utilizes hybridization capture-based target enrichment for superior specificity and uniform coverage.
  • Multi-Sample Compatibility: Designed for DNA from FFPE tissue, fresh tissue, and blood, enabling flexibility in sample types.
  • Guideline-Driven Gene Selection: Genes curated based on AMP/ASCO/CAP recommendations for clinically actionable insights.

Panel Performance

Features Performance
Coverage uniformity (0.2X) >99%
Reproducibility (%) 97.9
Sensitivity (%) 96.7
On Target Ratio (%) 75-85

Panel Specifications


Number of Genes: 53
Gene count/family: 53
Target Size: 0.17 Mb
Covered Regions: Whole CDS
Mutation Types: SNV / InDels / CNVs
Sample Type: Blood / FFPE
Platform Compatibility: Illumina, MGI, Thermo Fisher, Element Biosciences

PROCESS WORKFLOW

OncoCheck workflow

Data Analysis and Reporting


Precision Enrichment Delivering Superior On-Target Performance
oncoCheck NGS Assay

High-Precision On-Target Precision Across Samples:

On-target ratio performance of the OncoCheck NGS Assay across samples maintains a high on-target alignment (ranging from 76.0% to 79.5%), reflecting exceptional assay design, optimized enrichment, and reliable sequencing accuracy for comprehensive genomic profiling.

Unwavering Uniformity in OncoCheck Assay Performance
oncoCheck NGS Assay

Consistent Coverage Across Cancer Patients Sample:

Uniformity of OncoCheck NGS Assay coverage across samples consistently achieves 100% coverage at 0.2X depth, demonstrating exceptional assay design and reproducibility for reliable genomic analysis.

Ordering Information


Commercial Name Cat No. Pack Size Platform
OncoCheck NGS Test Kit (HRR) G710002-1 24 T Illumina
G710002-2 96 T Illumina
G710002-3 96 T – EZY Illumina – EZY
OncoCheck NGS Test Kit (HRR) G710002-4 24 T MGI
G710002-5 96 T MGI
G710002-6 96 T – EZY MGI – EZY
OncoCheck NGS Test Kit (HRR) G710002-7 24 T Aviti
G710002-8 96 T Aviti
G710002-9 96 T – EZY Aviti – EZY
OncoCheck NGS Test Kit (HRR) G710002-10 24 T Thermo
G710002-11 96 T Thermo
G710002-12 96 T – EZY Thermo – EZY

Resources


Download useful documents and technical information for OncoCheck NGS Panel.

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