RapiCycler-96

Real Time PCR System
Rapi cycler 96

Overview


RapiCycler 96 – Advanced Real-Time PCR Performance with Maximum Flexibility

RapiCycler 96 is engineered to meet the demanding requirements of advanced molecular laboratories. Available with 4 or 6 fluorescence channel configurations, capable of providing flexibility needed for diverse assay designs and multiplex workflows.

Designed for efficiency and precision, the system processes up to 96 samples per run, making it ideal for high-throughput testing environments. It supports a comprehensive range of applications, including qualitative and quantitative analysis, absolute and relative quantification, melting curve analysis, standard curve and dissociation curve analysis, multiplex PCR, SNP genotyping, high-resolution melting, allele discrimination, and temperature gradient optimization.

Powered by advanced semiconductor-based thermal control technology, RapiCycler 96 ensures rapid ramp rates and exceptional temperature uniformity for consistent amplification performance. Its high-sensitivity photoelectric detection system enables accurate real-time fluorescence monitoring across all channels. Combined with robust, intuitive, easy to use analysis software, the system delivers precise, reproducible, and reliable results for both research and diagnostic applications.

Features


  • Unique Side Scan Technology: Detects all fluorescent channels simultaneously, scanning 96 wells within just 5 seconds.
  • Ultra-Fast Ramp Rate: Up to 8°C/s, enabling faster run times without compromising accuracy.
  • Temperature Gradient Function: 12-column gradient with 0.1°C–42°C adjustable range for optimized assay conditions.
  • Automatic Hot Lid: Compatible with automated workstations for high-throughput workflows.
  • Built-in Audit Trail System: Hierarchical access and full traceability for regulatory compliance.
  • Energy-Efficient LED Light Source: Long service life, low maintenance, and environmentally friendly.
  • CE-IVD Certified: Validated for clinical diagnostic use.

Large 12.1” LCD Touch Screen

PC Operation

Automated Reporting

Automated Efficiency

Applications


Clinical Diagnostics

An open RT-PCR system enables highly sensitive and specific detection of viral, bacterial, and genetic targets. It supports validated diagnostic assays as well as laboratory-developed tests (LDTs), making it ideal for hospitals, reference labs, and public health facilities requiring flexible test menus.

Infectious Disease Detection

From respiratory pathogens to emerging outbreaks, the system delivers rapid and reliable pathogen identification. Its open platform allows laboratories to adapt quickly to new targets, ensuring preparedness for evolving public health challenges.

Oncology & Mutation Analysis

The instrument supports precise detection of gene mutations, expression profiling, and minimal residual disease monitoring. Its high sensitivity enables early detection of cancer-associated biomarkers, supporting personalized medicine and targeted therapy decisions.

Food & Environmental Testing

Designed for versatility, the platform detects microbial contamination and genetically modified organisms (GMOs) in food, water, and environmental samples. It ensures compliance with regulatory standards while maintaining throughput and reproducibility.

Research & Academic Applications

Ideal for gene expression studies, RNA quantification, and pathway analysis, the system provides researchers with flexibility in assay design. Its open chemistry format allows compatibility with a wide range of reagents and protocols.

Veterinary & Animal Health Testing

The instrument enables accurate detection of animal pathogens and supports herd health monitoring programs. Its adaptability makes it suitable for veterinary diagnostics, zoonotic disease surveillance, and livestock management.

Specifications


Model No. G2MRAPI4001 G2MRAPI4002
Sample Capacity 96 well, 12 × 8 strip, 96 × 0.2 ml single tube
Consumables 0.2 ml tube, 0.2 ml 8-tube strips, 0.2 ml 96-well plate
Reaction Volume 5–100 µl
Temperature Range 4–105 °C (Resolution: 0.1 °C)
Ramp Rate Up to 8.0 °C/s
Temperature Control Accuracy ±0.1 °C
Uniformity ±0.2 °C
Accuracy ±0.1 °C
Gradient 0.1–42 °C
Hot Lid Temperature 30–110 °C (Adjustable, default 105 °C)
Hot Lid Opening Mode Automatic cover opening
Temperature Control Block / Tube
Excitation Wavelength 400–710 nm
Emission Wavelength 500–810 nm
Detection Channels 4 Channels 6 Channels
Factory Calibrated Dyes F1: FAM / SYBR Green / EVA Green
F2: HEX / VIC / JOE / TET / YELLOW / NED
F3: ROX / Texas Red
F4: Cy5 / Quasar 670
F1: FAM / SYBR Green / EVA Green
F2: HEX / VIC / JOE / TET / YELLOW / NIC
F3: ROX / Texas Red
F4: Cy5 / Quasar 670
F5: Cy5.5 / Quasar 705
F6: CY3 / NED / TAMRA
Excitation Source Long-life LED
Detection Method High-sensitivity photoelectric detector
Dynamic Range 1–1010 copies
Sensitivity 1 copy
Feature Functions Qualitative/absolute quantification, relative quantitative, genotyping, HRM, melting curve, multiplex PCR, standard curve, relative standard curve, comparative Ct, allele identification/SNP genotyping, temperature gradient function, dissociation curve analysis, pathogen detection, plus/minus assays etc.
Special Temperature Functions Supports thermal gradient PCR, Long PCR, Touch Down PCR
Control Mode 12.1 inch adjustable colour touch screen control or PC control
Data Export Formats Excel, CSV, TXT, PDF
Reporting Automated report generation at the end of the run, ready for printing
Communication Ports USB 2.0, RS232
PC Operating System Windows 7, Windows 10
Dimensions (L × W × H) 520 × 350 × 330 mm
Net Weight 30 kg
Power Supply & Consumption AC 100–240 V, 50–60 Hz; 1500 W
Powerful software Pre-calibrated with optimized algorithms to calculate crosstalk coefficients, ensuring minimal interference from overlapping fluorescence dyes accurate detection
Data Collection Synchronous multi-channel detection with side-scan technology captures fluorescence data for 96 samples in 5 seconds. All channels are collected by default, with post-run reconfiguration and reanalysis available

Automated Results Interpretation & Analysis


Relative Quantification Analysis
Bar Chart – Relative Quantification Analysis

Relative gene expression analysis across multiple samples using ΔΔCt method.

Standard Curve
Standard Curve

Standard curve demonstrating high amplification efficiency and linear quantification.

Melting Curve
Melting Curve

Melting curve confirming specificity of PCR products through peak analysis.

Allelic Discrimination Plot
Allelic Discrimination Plot

Allelic discrimination plot for SNP genotyping and sample classification.

Ordering Information


CAT #

DESCRIPTION

G2MRAPI4001

RapiCycler 96 Real Time PCR System (4 channel)

G2MRAPI4002

RapiCycler 96 Real Time PCR System (6 channel)

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Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.


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