Jak2-Q Real Time PCR Kit

Qualitative Detection of JAK2 V617F Mutation with Wild Type Differentiation

The Jak2-Q Real Time PCR Kit is a CE-IVD molecular diagnostic test designed for the sensitive detection of the JAK2 V617F point mutation, a critical molecular marker in myeloproliferative neoplasms (MPNs).

This assay supports accurate molecular confirmation and classification of MPNs such as Polycythemia Vera (PV), Essential Thrombocythemia (ET), and Primary Myelofibrosis (PMF), enabling reliable mutation detection real-time PCR.

Product Overview

The Jak2-Q Real Time PCR Kit is an in vitro diagnostic (IVD) assay based on real-time PCR technology for the qualitative detection of JAK2 V617F mutation in DNA extracted from clinical blood samples.

The assay is a single-tube multiplex real-time PCR test, designed to detect:

  • Wild Type (WT) JAK2 gene
  • Mutant (V617F) JAK2 allele

This allows clear differentiation between WT positive, Mutant positive and Heterozygous mutation status, making it ideal for routine MPN molecular testing.

Why JAK2 V617F Testing Matters

The JAK2 V617F mutation is one of the most clinically relevant mutations in myeloproliferative neoplasms (MPNs).

It is reported to occur in:

Detecting JAK2 V617F supports:

Targets Detected

Mutation Target
  • JAK2 V617F point mutation
Wild Type Target
  • Wild type JAK2 gene
    (Multiplex assay detects both WT and MUT alleles for accurate interpretation.)

Applications

The kit is ideal for clinical molecular laboratories performing:

Jak2-Q
  • JAK2 V617F mutation testing
  • Molecular confirmation of myeloproliferative neoplasms (MPNs)
  • Classification support for PV, ET and PMF
  • Routine hematology mutation screening using real-time PCR
  • Integration into oncology and hematopathology molecular workflows

Key Features & Benefits

Sensitive JAK2 V617F Mutation Detection

Designed for reliable qualitative detection of the clinically significant V617F mutation in peripheral blood DNA.

Multiplex Real-Time PCR for WT & Mutant Differentiation

allowing confident genotype interpretation (WT / Mut / Heterozygous).

High Specificity with Probe-Based Detection

Fluorescent probe detection supports clear amplification interpretation

Clear Result Interpretation

Straightforward reporting enables fast conclusions

Sample Types & Reporting Output

Sample Types

Validated for use with:

  • Peripheral blood samples (EDTA)
Reporting Output

The test provides clear actionable output for clinicians and labs, including:

  • Positive / Negative interpretation
  • Ct values and amplification curves
  • WT and Mutant allele call
  • Heterozygous mutation reporting
  • Export options (USB / Bluetooth; system dependent) Jak2-Q Real Time PCR Kit_(CE-IV…)

Compatible System

OnePCR-Rapi-Q Ultra-Fast POC System

Designed for rapid molecular workflow and real-time PCR analysis.

OnePCR® workflow
  • Collect EDTA blood sample
  • Load 200 µl sample into Cartridge-1
  • Reconstitute Cartridge-2 reagents using provided buffer
  • Load cartridges into the device
  • Automated extraction + real-time PCR amplification
  • Results displayed as Positive/Negative with Ct values & curves

Storage & Stability

Ordering Information

Commercial Name Old Cat No. New Cat No. Pack Size
Jak2-Q Real Time PCR Kit G2M802621R G610029-3 24 Tests

Why Choose the Jak2-Q Real Time PCR Kit?

If your lab requires a reliable JAK2 V617F PCR kit for MPN molecular testing, the Jak2-Q kit delivers:

  • sensitive detection of JAK2 V617F mutation
  • multiplex differentiation of WT and Mutant alleles
  • strong assay confidence for heterozygous samples
  • CE-IVD workflow suitability for clinical molecular laboratories
  • compatibility with fast Genes2Me PCR systems for streamlined testing

Resources to get started

Download useful documents and technical information for the JAK-2-Q Real Time PCR Kit.

Product Brochure
IFU (Instructions for Use)
Report File

Product Enquiry Form

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Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.

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