HLA B27-Q Real-Time PCR Kit | Oncology POCT Testing

Product Overview

The HLA B27-Q Real-Time PCR Kit is an in vitro diagnostic (IVD) assay based on real-time PCR technology for the qualitative detection of HLA-B27 allele DNA in human clinical samples.

The test identifies HLA-B27 sequences encoded within the B locus of the Major Histocompatibility Complex (MHC) on chromosome 6.

The assay includes:

Target-specific primers and probes for HLA-B27 allele detection
Internal control targeting the human RNaseP gene (assay validity)

This ensures reliable reporting for autoimmune disease risk assessment and clinical correlation.

Why HLA-B27 Testing Matters

HLA-B27 is a Class I surface antigen strongly associated with inflammatory autoimmune diseases.

Clinical significance includes:

~90% of ankylosing spondylitis (AS) patients are HLA-B27 positive
Associated with: Psoriatic arthritis | Reactive arthritis | Inflammatory bowel disease–related arthritis

HLA-B27 testing supports:

Early detection of spondyloarthropathies
Differential diagnosis in chronic back pain
Risk stratification in symptomatic individuals
Clinical decision support alongside imaging and serology

Targets Detected

Genetic Target

HLA-B27 allele
(B locus, MHC region — Chromosome 6)

Internal Control

Human RNaseP gene
Ensures DNA quality and assay performance validation.

Applications

The kit is ideal for clinical molecular laboratories and rheumatology diagnostics performing:

HLA-B27 genetic testing
Ankylosing spondylitis risk assessment
Spondyloarthropathy screening
Autoimmune disease differential diagnosis
Chronic inflammatory back pain evaluation
Integration into immunogenetic testing workflows

Key Features & Benefits

Accurate HLA-B27 Allele Detection

Designed for sensitive and specific identification of the HLA-B27 genetic marker linked to autoimmune rheumatologic disorders.

Multiplex Real-Time PCR Assay

Simultaneous detection of HLA-B27 target DNA and RNaseP internal control ensuring confident result interpretation.

Fluorescent Probe-Based Detection

for specific and sensitive target detection

Clear Result Interpretation

Straightforward reporting enables fast conclusions

Sample Types & Reporting Output

Sample Types

Validated for use with:

Whole blood
Serum
Plasma

Reporting Output

The test provides clear, clinician-ready output, including:

Positive / Negative HLA-B27 status
Ct values
Amplification curves
Internal control validation
Exportable reports via USB / Bluetooth (system dependent)

Compatible System

OnePCR-Rapi-Q Ultra-Fast POC System

Designed for automated workflow including extraction and amplification.

Collect blood / plasma / serum sample
Load 200 µl sample into Cartridge-1
Reconstitute Cartridge-2 reagents
Insert cartridges / tubes into system
Automated DNA extraction + Real-Time PCR
Results displayed as Positive/Negative with Ct curves

Storage & Stability

Store at 2°C – 8°C
Shelf-life: 12 months
Protect from direct sunlight
Ship and handle under controlled temperature conditions

Commercial Name	Old Cat No.	New Cat No.	Pack Size
HLA B27-Q Real-Time PCR Kit	G2M801121R	G610046-3	24 Tests

Commercial Name

Old Cat No.

New Cat No.

Pack Size

HLA B27-Q Real-Time PCR Kit

G2M801121R

G610046-3

24 Tests

Why Choose the HLA B27-Q Real-Time PCR Kit?

If your lab requires a reliable HLA-B27 PCR test kit for autoimmune disease risk assessment, this assay delivers:

accurate HLA-B27 allele detection
internal control-validated results
multiplex probe-based PCR chemistry
compatibility with rapid POC PCR systems
streamlined workflow for immunogenetic testing

Resources to get started

Download useful documents and technical information for the HLA-B27 Real-Time PCR Kit.

Product Brochure

IFU (Instructions for Use)

Report File

Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.

Qualitative Detection of HLA-B27 Allele for Ankylosing Spondylitis & Spondyloarthropathies

Product Overview

Why HLA-B27 Testing Matters