Sepsis-Q Real Time PCR Kit

Multiplex Detection of Sepsis Pathogens by Real-Time PCR

The Sepsis-Q Real Time PCR Kit is a CE-IVD molecular diagnostic assay designed for the qualitative detection of sepsis-causing pathogens in clinical samples.

This multiplex assay enables simultaneous identification of bacterial and fungal pathogens associated with bloodstream infections, supporting rapid diagnosis, early intervention, and improved patient outcomes through a single real-time PCR workflow.

Product Overview


The Sepsis-Q Real Time PCR Kit is an in vitro diagnostic (IVD) assay based on advanced real-time PCR technology for detection of sepsis-related pathogens directly from whole blood and blood culture samples.

The assay integrates automated nucleic acid extraction, multiplex real-time PCR amplification and Fluorescent probe-based detection within a fully automated workflow on compatible systems.

The kit is designed as a 4-tube multiplex assay, enabling simultaneous detection of 20 clinically relevant sepsis pathogens along with an internal control, ensuring comprehensive coverage and reliable results.

Sepsis-Q

Why Sepsis Molecular Testing Matters


Sepsis is a life-threatening condition caused by the body’s extreme response to infection, which can rapidly progress to septic shock, organ failure, and death if not diagnosed early.

Common sources of infection include:

Rapid and accurate pathogen identification is critical because:

Real-time PCR enables rapid and sensitive detection of pathogens, helping clinicians initiate timely and appropriate treatment.

Pathogens Detected


Bacterial Targets
  • Staphylococcus aureus
  • Human Parechovirus (HPeV)
  • Coagulase-negative staphylococci (CoNS: S. epidermidis, S. haemolyticus)
  • Escherichia coli
  • Klebsiella pneumoniae
  • Pseudomonas aeruginosa
  • Acinetobacter baumannii
  • Enterococcus faecalis
  • Enterococcus faecium
  • Streptococcus pneumoniae
  • Streptococcus spp. (S. pyogenes, S. agalactiae)
  • Listeria monocytogenes
  • Stenotrophomonas maltophilia
Fungal Targets
  • Candida albicans
  • Non-albicans Candida species (C. parapsilosis, C. tropicalis, C. krusei)
  • Aspergillus fumigatus
Internal Control
  • Human RNaseP gene ensuring sample integrity and assay performance

Applications


The kit is ideal for clinical molecular laboratories performing:

Sepsis-Q
  • Sepsis molecular diagnostics
  • Bloodstream infection detection
  • Critical care and ICU diagnostics
  • Hospital emergency testing
  • Antimicrobial stewardship programs
  • Infection control and surveillance

Key Features & Benefits


Comprehensive 20-Pathogen Sepsis Panel

Simultaneous detection of major bacterial and fungal pathogens associated with sepsis.

4-Tube Multiplex Real-Time PCR Design

Optimized multiplexing enables accurate differentiation of multiple targets.

Rapid Detection for Critical Care

Supports early diagnosis and faster clinical decision-making in sepsis management.

Probe-Based Detection Technology

Ensures high specificity and reliable amplification monitoring.

Automated Extraction-to-Result Workflow

Fully integrated system reduces hands-on time and minimizes error.

High Sensitivity and Specificity

Designed for reliable clinical performance in critical diagnostic settings.

Sample Types & Reporting Output


Sample Types

Validated for use with:

  • Whole blood samples (with preprocessing)
  • Blood culture samples

Samples should be collected and processed according to recommended protocols to ensure optimal performance.

Reporting Output

The assay provides clear qualitative reporting, including:

  • Pathogen-specific Positive / Negative results
  • Ct values for each detected target
  • Amplification curves
  • Internal control validation
  • Multiplex interpretation across all targets
  • Exportable reports via USB or Bluetooth

Compatible System


OnePCR-Rapi-Q Ultra-Fast POC System
OnePCR® workflow
  • Collect whole blood / blood culture sample
  • Perform preprocessing for whole blood (pathogen enrichment step)
  • Load 200 µl sample into Cartridge-1
  • Reconstitute Cartridge-2 (RM1–RM4) reagents
  • Insert cartridges into the system
  • Automated DNA extraction + multiplex PCR
  • Results displayed as pathogen-specific Positive/Negative with Ct curves

Storage & Stability


Ordering Information


Commercial Name Old Cat No. New Cat No. Pack Size
Sepsis-Q Real Time PCR Kit G2M804026 G610027-3 24 Tests

Why Choose the Sepsis-Q Real Time PCR Kit?


If your laboratory requires a reliable sepsis multiplex PCR solution, this assay delivers:

  • detection of 20 critical sepsis pathogens in one test
  • rapid molecular diagnosis for time-sensitive conditions
  • combined bacterial and fungal pathogen coverage
  • high-performance probe-based PCR chemistry
  • internal control validated results
  • automated extraction-to-result workflow
  • suitability for ICU, hospital, and reference laboratory settings

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Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.


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