MCP-Q-Comprehensive Real-Time PCR Kit - Genes2Me™

Product Overview

The MCP-Q Comprehensive Real Time PCR Kit is an in vitro diagnostic (IVD) assay based on advanced real-time PCR technology for broad-spectrum detection of meningitis pathogens.

The assay integrates automated nucleic acid extraction, multiplex real-time PCR amplification and Fluorescent probe-based detection within a fully automated workflow on compatible systems.

The kit is designed as a 4-tube multiplex assay, enabling simultaneous detection of 16 meningitis-causing pathogens along with an internal control, ensuring high diagnostic coverage and assay reliability.

Why Comprehensive Meningitis Testing Matters

Meningitis is a serious and potentially life-threatening condition involving inflammation of the meninges surrounding the brain and spinal cord.

Common symptoms include:

Severe headache
Fever
Neck stiffness
Sensitivity to light
Nausea and vomiting
Altered mental status

Accurate and rapid diagnosis is critical because:

Bacterial meningitis can be fatal if untreated
Viral and fungal causes require different management strategies
Early identification improves survival and reduces complications
Traditional methods (culture/microscopy) are time-consuming and less sensitive

Multiplex real-time PCR enables fast, sensitive, and specific detection of multiple pathogens from limited sample volumes, supporting urgent clinical decision-making.

Pathogens Detected

Viral Targets

Japanese Encephalitis Virus (JEV)
Human Parechovirus (HPeV)
Herpes Simplex Virus 1 & 2 (HSV-1, HSV-2)
West Nile Virus
Cytomegalovirus (CMV)
Varicella-Zoster Virus (VZV)
Epstein-Barr Virus (EBV)
Adenovirus
Enterovirus

Bacterial Targets

Streptococcus pneumoniae
Neisseria meningitidis
Streptococcus agalactiae
Escherichia coli K1
Haemophilus influenzae
Listeria monocytogenes

Fungal Targets

Cryptococcus neoformans / Cryptococcus gattii

Internal Control

Human biological internal control ensuring sample integrity and assay performance

Applications

The kit is ideal for clinical molecular laboratories performing:

Meningitis molecular diagnostics
Syndromic CNS infection testing
Rapid pathogen identification in suspected meningitis
Hospital emergency and critical care testing
Infectious disease diagnostics
Public health surveillance

Key Features & Benefits

Comprehensive 16-Pathogen Meningitis Panel

Simultaneous detection of major viral, bacterial, and fungal meningitis pathogens.

4-Tube Multiplex Real-Time PCR Design

Optimized assay structure for accurate target differentiation and high performance.

Syndromic Testing Approach

Enables rapid differential diagnosis without multiple individual assays.

Probe-Based Detection for High Specificity

Fluorescent probe chemistry ensures precise and reliable pathogen identification.

Automated Extraction-to-Result Workflow

Seamless integration with the OnePCR system reduces hands-on time and errors.

Rapid Turnaround for Critical Conditions

Supports urgent clinical decisions in suspected meningitis cases.

Sample Types & Reporting Output

Sample Types

Validated for use with:

Cerebrospinal fluid (CSF) (primary sample)
Whole blood
Plasma

Samples should be transported promptly and processed as per recommended guidelines to maintain nucleic acid integrity.

Reporting Output

The assay provides clear qualitative reporting, including:

Pathogen-specific Positive / Negative results
Ct values for each detected target
Amplification curves
Internal control validation
Multiplex interpretation across all targets
Exportable reports via USB or Bluetooth

Compatible System

OnePCR-Rapi-Q Ultra-Fast POC System

Collect CSF / blood / plasma sample
Perform preprocessing (if required for whole blood)
Load 200 µl sample into Cartridge-1
Reconstitute Cartridge-2 (RM1–RM4) reagents
Insert cartridges into the system
Automated nucleic acid extraction + multiplex PCR
Results displayed as pathogen-specific Positive/Negative with Ct curves

Storage & Stability

Store at 2°C – 8°C (Cartridge reagents)
Certain components stored at controlled conditions as specified
Ship at 10°C – 30°C
Shelf-life: 12 months
Protect from direct sunlight
Do not use beyond expiry date

Commercial Name	Old Cat No.	New Cat No.	Pack Size
MCP-Q comprehensive Real Time PCR Kit for Meningitis	G2M803821	G610038-3	24 Tests

Why Choose the MCP-Q Comprehensive Real Time PCR Kit?

If your laboratory requires a reliable meningitis multiplex PCR panel, this assay delivers:

detection of 16 key meningitis pathogens in one test
combined viral, bacterial, and fungal coverage
rapid syndromic testing for critical care scenarios
high-sensitivity probe-based PCR performance
internal control validated results
automated extraction-to-result workflow
suitability for hospital, reference, and infectious disease laboratories

Multiplex Detection of Meningitis Pathogens by Real-Time PCR

Product Overview