B. Pertussis-Q Real-Time PCR Kit

Product Overview

Whooping cough (pertussis) is one of the most underdiagnosed respiratory diseases in clinical settings - its early symptoms mirror a common cold, making timely identification a significant challenge. Caused by Bordetella pertussis, a highly contagious gram-negative bacterium, the disease progresses rapidly from mild cough to severe paroxysmal coughing fits and can be life-threatening in infants and young children. Despite widespread vaccination, pertussis remains a global health concern, making early and accurate detection critical for effective treatment and outbreak prevention.

The B. Pertussis-Q Real-Time PCR Kit from Genes2Me delivers a definitive, lab-grade answer in a single run. It detects B. pertussis-specific DNA directly from nasopharyngeal and oropharyngeal swabs - with no ambiguity, no repeat runs, and no compromise on accuracy.

Targets:
Bordetella pertussis
Technology:
TaqMan-based Real-Time PCR
Detection:
Qualitative (Detected / Not Detected)
Sample Type:
Nasopharyngeal & Oropharyngeal Swabs
Certification:
CE-IVD
Pertussis-Q

Genes2Me is a CE Marked molecular diagnostics company trusted by clinical laboratories across global markets. The B. Pertussis-Q Kit meets European conformity standards for in vitro diagnostic devices — validated to international benchmarks for accuracy, reliability, and safety.

How the Assay Works

The B. Pertussis-Q kit utilizes advanced real-time PCR technology with a multiplex design to simultaneously detect B. pertussis-specific DNA and the internal control. The target pathogen is detected in the FAM channel, while RNaseP serves as an internal control in the HEX channel, minimizing the risk of false negatives and guaranteeing accurate results. A positive control template is included to validate assay performance in every run.

Why Multiplex Testing Matters

Pertussis infections present with non-specific early symptoms that overlap significantly with other respiratory pathogens, making differential diagnosis challenging. Multiplex testing enables simultaneous pathogen detection with built-in controls, reducing false negatives and ensuring accurate, timely identification - critical for effective patient management and outbreak containment.

Sample Type & Reporting Output

Sample Type:
Nasopharyngeal and oropharyngeal swab samples
Reporting Output:
Qualitative detection of B. pertussis DNA (Detected / Not Detected)
Targets Detected:
Bordetella pertussis

Key Features & Benefits

Multiplex assay with internal control

Simultaneously detects B. pertussis DNA (FAM) and RNaseP internal control (HEX) - reducing false negatives and ensuring result integrity.

High sensitivity with cut-off Ct value of 35

Detects bacterial DNA even at very low quantities, enabling confident identification in the early stages of infection.

All-inclusive reagent kit

Contains specific primers, fluorescent probes, Taq polymerase, positive control template, and qPCR master mix - streamlining lab workflows.

Rapid turnaround

Delivers results within hours, offering a far faster and more sensitive alternative to traditional culture or microscopy methods.

Compatible with multiple leading PCR platforms

Validated on RapiCycler 96, Bio-Rad CFX96, QuantStudio™ 5, and LightCycler® 480.

Simple workflow with stable storage conditions

Minimal hands-on time and straightforward setup mean faster lab onboarding and lower training overhead.

Applications

  • Clinical & Hospital Laboratories: Enables rapid, sensitive detection of B. pertussis from respiratory swabs, supporting timely treatment decisions and reducing the risk of disease spread.
  • Paediatric & Neonatal Care: Critical early detection tool for infants and young children, where pertussis complications including pneumonia, seizures, and respiratory failure can be life-threatening.
  • Outbreak Surveillance: Supports rapid identification and containment of pertussis clusters in hospitals, schools, and community settings, even when vaccination coverage is high.
  • Immunocompromised Patients: Essential diagnostic support for patients at heightened risk of severe outcomes, where early and accurate detection is critical to clinical management.

Analytical Performance

  • High analytical sensitivity and specificity
  • Cut-off Ct value of 35 for positive detection - reliable even at very low bacterial DNA concentrations
  • No cross-reactivity with common respiratory pathogens including Influenza A/B, RSV, and SARS-CoV-2

Ordering Information

Commercial Name Old Cat No. New Cat No. Pack Size
B. Pertussis-Q Real-Time PCR Kit G610002-1 50 Tests

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Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.

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