MERS-Q Real-Time PCR Kit

Product Overview


Middle East Respiratory Syndrome (MERS) is a severe respiratory illness caused by MERS-CoV, first identified in Saudi Arabia in 2012. With a mortality rate of approximately 35%, it ranks among the most lethal respiratory viruses known. Symptoms range from mild flu-like illness to severe pneumonia and respiratory failure, and a significant proportion of cases are asymptomatic, making clinical diagnosis alone unreliable. The absence of early, accurate detection creates serious risks for healthcare settings and communities where the virus can spread undetected.

The MERS-Q Real-Time PCR Kit from Genes2Me delivers sensitive, specific detection of MERS-CoV RNA in a single-tube assay. It detects viral RNA directly from nasopharyngeal and oropharyngeal swabs collected in VTM, providing dependable results quickly to support timely clinical decisions and effective outbreak containment.

Targets:
MERS Coronavirus (MERS-CoV RNA)
Technology:
TaqMan-based Real-Time RT-PCR
Detection:
Qualitative (Detected / Not Detected)
Sample Type:
Nasopharyngeal & Oropharyngeal Swabs in VTM
Certification:
CE-IVD
MERS-Q

Genes2Me is a CE Marked molecular diagnostics company trusted by clinical laboratories across global markets. The MERS-Q Kit meets European conformity standards for in vitro diagnostic devices, validated to international benchmarks for accuracy, reliability, and safety.

How the Assay Works


The MERS-Q kit uses a single-tube real-time RT-PCR design with MERS-specific primers and fluorescent probes for accurate target detection. MERS-CoV RNA is detected in the FAM channel, while the HEX channel monitors an internal control to validate extraction and amplification in every run. Positive and no-template controls are included to guarantee assay accuracy and eliminate contamination risks across all workflows.

Why Multiplex Testing Matters


MERS-CoV infections frequently present with non-specific symptoms indistinguishable from other respiratory illnesses, and asymptomatic transmission adds further diagnostic complexity. Multiplex testing with a built-in internal control enables simultaneous pathogen detection and run validation, reducing false negatives and ensuring accurate, timely identification. In a disease with a 35% mortality rate, the cost of a missed diagnosis is too high to leave to clinical judgement alone.

Sample Type & Reporting Output


Sample Type:
Nasopharyngeal and oropharyngeal swabs collected in VTM
Reporting Output:
Qualitative detection of MERS-CoV RNA (Detected / Not Detected)
Targets Detected:
MERS Coronavirus (MERS-CoV RNA)

Key Features & Benefits


Multiplex assay with internal control

Simultaneously detects MERS-CoV RNA (FAM) and validates the run via internal control (HEX), ensuring reliable results even under challenging laboratory conditions.

High sensitivity with Ct cut-off of 35 or below

Detects viral RNA at very low concentrations, enabling confident identification in early-stage, low-titre, and asymptomatic infections.

Positive and no-template controls included

Both controls are supplied in every kit, guaranteeing assay accuracy and eliminating contamination risks.

Compatible with multiple leading PCR platforms

Validated on RapiCycler 96, Bio-Rad CFX96, QuantStudio 5, and LightCycler 480.

Streamlined workflow with optimised reagent system

Carefully formulated reagents and optimised reaction conditions minimise preparation time and reduce the potential for procedural errors.

High reproducibility across runs

Consistent, trustworthy results support reliable clinical decision-making and seamless integration into existing laboratory workflows.

Applications


  • Clinical & Hospital Laboratories: Enables rapid, sensitive detection of MERS-CoV RNA from swab samples in VTM, supporting timely clinical decisions and effective infection control measures.
  • Paediatric & Neonatal Care: Supports early identification in younger patients, where MERS presentations may be atypical or asymptomatic, ensuring no case is missed.
  • Outbreak Surveillance: Critical tool for rapid identification and containment of MERS-CoV clusters, particularly in healthcare settings and regions with known zoonotic exposure risks.
  • Immunocompromised Patients: Essential for patients at heightened risk of severe outcomes, where early and accurate detection of MERS-CoV is critical to clinical management and isolation protocols.
  • Travel & Occupational Health: Reliable screening in travel medicine clinics and occupational health programmes, particularly for individuals returning from or working in MERS-endemic regions.

Analytical Performance


  • High analytical sensitivity and specificity
  • Ct cut-off of 35 or below for positive detection, reliable even at very low viral RNA concentrations
  • No cross-reactivity with common respiratory pathogens
  • Positive and no-template controls included for full assay integrity validation

Ordering Information


Commercial Name Old Cat No. New Cat No. Pack Size
MERS-Q Real Time PCR Kit G2M802121R 50 Tests

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Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.


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