As COVID-19 continues to circulate globally, rapid and reliable molecular diagnosis remains essential for effective patient management, infection control, and public health surveillance. The emergence of viral mutations and varying clinical presentations, from asymptomatic infection to severe pneumonia, ARDS, sepsis, and death has highlighted the limitations of single-target molecular assays. Accurate multi-gene detection strategies are therefore critical to minimise false-negative results and maintain diagnostic confidence across evolving variants.
The RT-Direct Multiplex Real Time PCR Kit for COVID-19 from Genes2Me provides rapid and precise qualitative detection of SARS-CoV-2 by simultaneously targeting the RdRp, E, and N genes in a single multiplex assay. Designed with an innovative direct sample processing workflow, the kit incorporates Buffer R3, eliminating the need for a separate viral RNA extraction kit, significantly reducing turnaround time, workflow complexity, and operational costs.
The RT-Direct Multiplex Real Time PCR Kit uses multiplex real-time reverse-transcription PCR technology to simultaneously detect three SARS-CoV-2 genomic targets: the RdRp gene, E gene, and N gene. A unique feature of the assay is Buffer R3, which enables direct RNA release from clinical samples without requiring a separate viral RNA extraction procedure. The buffer safely inactivates pathogenic samples while preserving and stabilizing released viral RNA, and thus reducing hands-on processing time. Guanidine thiocyanate present in Buffer R3 disrupts the viral capsid, rendering the virus non-infectious while maintaining nucleic acid integrity for amplification.
An integrated internal control monitors PCR inhibition, helping prevent false-negative results. The kit also includes a positive control template and all essential reagents required for seamless assay setup and performance validation.
As SARS-CoV-2 continues to evolve, reliance on a single genetic target may increase the risk of missed detection due to mutations in the viral genome. Multiplex testing overcomes this challenge by simultaneously targeting the RdRp, E, and N genes, ensuring robust and reliable detection even if mutations affect one target region.
The inclusion of multiple viral targets improves analytical sensitivity and specificity while strengthening confidence in clinical reporting. The E gene supports sensitive screening, the RdRp gene provides highly specific confirmation of SARS-CoV-2, and the N gene enhances detection of low viral load samples due to its high expression levels.
Simultaneous detection of RdRp, E, and N genes ensures high analytical sensitivity and specificity while minimizing the risk of variant-related false-negative results.
Unique Buffer R3 eliminates the requirement for a separate viral RNA extraction kit, simplifying sample processing, reducing turnaround time, and lowering testing costs.
Validated on multiple real-time PCR systems including QuantStudio 5 Real-Time PCR System, CFX96 Real-Time PCR System, LightCycler 480 System and RapiCycler 96 Real Time PCR System
Provides dependable qualitative SARS-CoV-2 detection with a Ct cut-off value of ≤35, including samples with low viral loads.
Supplied with primer and probe mix, qRT-PCR master mix, Buffer R3, positive control template, and RNase/DNase-free water for a streamlined and contamination-controlled workflow.
RT-Direct Multiplex PCR specific amplification plot of Treponema pallidum Positive Control (HEX Channel) along with Internal Control (Cy5 Channel)
| Commercial Name | Old Cat No. | New Cat No. | Pack Size |
|---|---|---|---|
| RT-Direct Multiplex Real Time PCR Kit | G2M020321 | G2M020321 | 50 Tests |
Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.
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