RCP-Q Comprehensive Real Time PCR Kit

Product Overview

Respiratory tract infections are among the leading causes of morbidity worldwide and may be caused by a wide range of viral pathogens presenting with highly overlapping clinical symptoms. Patients commonly exhibit fever, cough, sore throat, nasal congestion, breathing difficulty, bronchitis, or pneumonia, making syndromic diagnosis challenging without molecular confirmation. Rapid and accurate identification of respiratory pathogens is critical for timely clinical management, infection control, outbreak monitoring, and appropriate therapeutic decisions.

The RCP-Q Comprehensive Real Time PCR Kit for Respiratory from Genes2Me is a highly multiplexed CE-IVD molecular diagnostic assay designed for the qualitative detection of 28 major respiratory pathogens in human clinical samples. The assay utilizes real-time PCR technology across all tube workflow, enabling simultaneous detection and differentiation of multiple respiratory viruses associated with upper and lower respiratory tract infections.

Targets:

Influenza A, Influenza B, RSV A, RSV B, Human Metapneumovirus (HMPV), Rhinovirus, Enterovirus, Bocavirus, Adenovirus, Parechovirus, Parainfluenza Virus 1, Parainfluenza Virus 2, Parainfluenza Virus 3, Parainfluenza Virus 4, Streptococcus pneumoniae, Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila, Bordetella pertussis, Klebsiella pneumoniae, Streptococcus pyogenes, Corynebacterium diphtheriae, Corynebacterium ulcerans, Haemophilus influenzae, Bordetella parapertussis (BPP), Group C Streptococci, Group G Streptococci, Measles virus

Technology:

Real-Time PCR (Multiplex TaqMan Assay)

Detection:

Qualitative (Detected / Not Detected)

Sample Type:

Sputum, tracheal aspirate, nasopharyngeal aspirate, throat swab, nose swab, and nasopharyngeal swab

Certification:

CE-IVD

How the Assay Works

The RCP-Q Comprehensive assay is based on multiplex real-time PCR technology. The assay is organized into a multiplex format, where each Ready Mix contains optimized primers and probes for a defined pathogen panel along with an Internal/Exogenous Control for process validation and to ensure reliable results. The distinct fluorescent channels enable simultaneous detection of multiple targets within each reaction tube. The assay includes dedicated positive controls for each Ready Mix and negative controls for contamination monitoring.

Why Comprehensive Respiratory Molecular Testing Matters

Respiratory infections caused by viral pathogens often present with similar clinical manifestations, making differential diagnosis difficult using symptoms alone. Traditional diagnostic approaches may require multiple sequential tests, increasing turnaround time and delaying treatment decisions.

Comprehensive multiplex molecular testing enables rapid identification of multiple respiratory pathogens in a single workflow, supporting early and accurate diagnosis and appropriate patient isolation and infection control resulting in better management of immunocompromised and high- risk patients.

Sample Type & Reporting Output

Sample Type:

Sputum, tracheal aspirate, nasopharyngeal aspirate, throat swab, nose swab, and nasopharyngeal swab

Reporting Output:

Qualitative detection of respiratory pathogens (Detected / Not Detected)

Targets Detected:

Key Features & Benefits

Comprehensive 28-pathogen respiratory panel

Simultaneously detects 28 clinically relevant respiratory pathogens in a single workflow.

Optimized multiplex assay design

Efficient multiplex configuration minimizes time and reagent consumption while maintaining high analytical performance and target specificity.

Broad respiratory pathogen coverage

Covers major viral respiratory pathogens including Influenza A/B, RSV, SARS-CoV-2, endemic coronaviruses, Parainfluenza viruses, Rhinovirus, Adenovirus, Enterovirus, and more.

Built-in internal and exogenous controls

Integrated controls validate nucleic acid extraction, amplification efficiency, and sample integrity while helping identify PCR inhibition.

Compatible with leading PCR platforms

Validated on RapiCycler 96, Bio-Rad CFX96 Touch, QuantStudio 5, and LightCycler 480 systems for seamless laboratory integration.

Applications

Clinical & Hospital Laboratories: Supports rapid and comprehensive diagnosis of respiratory tract infections in symptomatic patients presenting with fever, cough, respiratory distress, or pneumonia.
Respiratory Infection Panels: Ideal for syndromic respiratory testing where multiple viral pathogens may present with similar clinical symptoms.
Outbreak Surveillance & Public Health: Enables rapid identification and monitoring of circulating respiratory pathogens during seasonal outbreaks and epidemic events.
Differential Diagnosis: Assists in distinguishing between Influenza, RSV, SARS-CoV-2, endemic coronaviruses, and other respiratory pathogens with overlapping symptoms.

Analytical Performance

Multiplex qualitative detection of 28 respiratory pathogens across all optimized reaction mixes
Ct cut-off value of ≤35 for positive detection
Dedicated Positive Controls included for all tubes Ready Mixes
Analytical sensitivity and specificity of 100%
Limit of detection as low as 100 copies/ml for highly sensitive molecular analysis
Excellent linearity with R² 0.999 across the quantification range
Clinical sensitivity of 99% and clinical specificity of 98%

Commercial Name	Old Cat No.	New Cat No.	Pack Size
RCP-Q Comprehensive Real Time PCR Kit	G2M803521	G610005	50 Tests

Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.

Product Overview

How the Assay Works

Why Comprehensive Respiratory Molecular Testing Matters

Sample Type & Reporting Output