Accurate and timely molecular diagnosis remains critical for controlling the spread of COVID-19 and enabling effective patient management. As SARS-CoV-2 continues to evolve, diagnostic assays must have high sensitivity and specificity across circulating variants while supporting rapid laboratory workflows. Clinical presentations can range from asymptomatic infection to severe pneumonia, acute respiratory distress syndrome (ARDS), sepsis, septic shock, and death, particularly in vulnerable and immunocompromised populations.
The ViralDtect-II Multiplex Real-Time PCR Kit for COVID-19 from Genes2Me is designed for rapid and reliable qualitative detection of SARS-CoV-2 by simultaneously targeting the RdRp, E, and N genes in a single multiplex RT-PCR assay. The assay delivers highly sensitive and specific detection from respiratory specimens including oropharyngeal swabs and sputum samples, supporting accurate diagnosis and differential diagnosis of suspected COVID-19 cases.
The ViralDtect-II Multiplex Real-Time PCR Kit simultaneously amplify and detect three SARS-CoV-2 genomic targets: the RdRp gene, E gene, and N gene by utilizing multiplex real-time reverse transcription PCR technology.
The assay includes an integrated Internal Control (HEX channel) to monitor the presence of PCR inhibitors within clinical specimens. This internal control significantly reduces the risk of false-negative results caused by sample-related inhibition or technical variability.
As SARS-CoV-2 continues to evolve, dependence on a single genetic target may increase the risk of missed detection due to mutations in the viral genome. Multiplex testing overcomes this challenge by simultaneously targeting the RdRp, E, and N genes, ensuring robust and reliable detection even if mutations affect one target region. Multiple viral targets detection improve analytical sensitivity and specificity while strengthening confidence in clinical reporting. The E gene supports sensitive screening, the RdRp gene provides highly specific confirmation of SARS-CoV-2, and the N gene enhances detection of low viral load samples due to its high expression levels.
Simultaneous detection of RdRp, E, and N genes improves analytical sensitivity and specificity.
Integrated HEX-labelled internal control monitors PCR inhibition, ensuring reliable assay performance.
Validated on widely used real-time PCR systems including QuantStudio 5 Real-Time PCR System, CFX96 Real-Time PCR System, LightCycler 480 System, and RapiCycler 96 Real Time PCR System.
Supplied with primer and probe mix, qRT-PCR master mix, positive control template, and RNase/DNase-free water for simplified assay preparation and workflow consistency.
cut-off value of ≤35 ensures accurate and dependable SARS-CoV-2 detection, including specimens containing low viral RNA concentrations.
ViralDtect-II Multiplex Real for Time PCR specific amplification plot of Treponema pallidum Positive Control (HEX Channel) along with Internal Control (Cy5 Channel)
| Commercial Name | Old Cat No. | New Cat No. | Pack Size |
|---|---|---|---|
| ViralDtect-II Multiplex Real for Time PCR Kit | G2M020220 | G2M020220 | 50 Tests |
Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.
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