The JAK2 V617F mutation (1849 G>T) is a critical biomarker in myeloproliferative neoplasms (MPNs), present in nearly all patients with polycythemia vera (PV) and in a substantial percentage of those with essential thrombocythemia (ET) and primary myelofibrosis (PMF) — diseases that significantly affect blood cell production and overall patient health. Early detection of this mutation is vital for accurate diagnosis, patient stratification, and effective treatment planning, enabling clinicians to initiate personalised treatment strategies and improve long-term patient outcomes.
To address this important clinical need, Genes2Me introduces the JAK2-Q Real-Time PCR Kit — an advanced molecular diagnostic solution designed for the rapid, accurate, and highly sensitive detection of the JAK2 V617F mutation in DNA extracted from clinical blood samples. With its optimised assay design and validated performance, the JAK2-Q Kit empowers laboratories and clinicians with a reliable, efficient, and non-invasive diagnostic tool to support early detection, guide treatment pathways, and monitor disease progression with confidence.
The JAK2-Q Kit utilises a Real-Time PCR system with TaqMan probe chemistry employing distinct fluorescent probes for simultaneous detection of both wild-type (FAM channel) and mutant (HEX channel) alleles within a single reaction. This dual-channel design provides robust internal control, minimises the risk of false-negative results, and enables detection of the JAK2 V617F mutation even at low allelic frequencies. Requiring only a standard blood sample, the assay delivers results within hours and eliminates the need for invasive procedures such as bone marrow biopsy or cytogenetic testing.
JAK2 V617F mutation testing is a cornerstone of MPN diagnosis and management. Detection of this mutation supports accurate disease classification across PV, ET, and PMF, enabling clinicians to differentiate MPNs from other haematological conditions and select appropriate targeted therapies. With a limit of detection of 0.3%, precision with CV less than 4.5%, and clinical accuracy of at least 96% sensitivity and at least 98% specificity, the JAK2-Q Kit provides laboratories with dependable molecular diagnostic performance for haematology diagnostics and disease monitoring.
Detects the JAK2 V617F mutation (1849 G>T) associated with polycythemia vera, essential thrombocythemia, and primary myelofibrosis.
LOD of 0.3%, precision with CV less than 4.5%, and clinical accuracy of at least 96% sensitivity and at least 98% specificity.
Distinct FAM-channel (wild-type) and HEX-channel (mutant) probes enable simultaneous allele detection, providing robust internal control and minimising false-negative results.
Requires only a standard blood sample, eliminating the discomfort and risks associated with bone marrow biopsy or cytogenetic testing.
Validated on leading Real-Time PCR platforms including Thermo Fisher QuantStudio™ 5, Bio-Rad CFX96, Roche LightCycler® 480, and Genes2Me RapiCycler 96.
Optimised assay design delivers results within hours, accelerating clinical decision-making and supporting timely therapeutic intervention.
With the JAK2-Q Real-Time PCR Kit, laboratories can achieve highly sensitive, reproducible, and clinically meaningful detection of the JAK2 V617F mutation. By supporting early diagnosis, disease monitoring, and improved clinical evaluation of myeloproliferative neoplasms, the kit helps clinicians make informed decisions for patient care and disease management. Empower your laboratory with the JAK2-Q Real-Time PCR Kit — because timely molecular insights can make a lasting difference in patient outcomes.
When the Ct value of the sample to be tested is outside the reference range and there is a typical S-shaped amplification curve, the test result is positive; When the Ct value of the sample to be tested is within the reference range, or the Ct value is outside the reference range but there is no typical S-shaped amplification curve, the test result is negative.
Channel 1 Slope: -3.392 Intercept: 39.372 Correlation: -0.998 Efficiency 3: 97.1430 Channel 3 Slope: -3.240 Intercept: 38.672 Correlation: -0.999 Efficiency 2: 103.52
| Commercial Name | Old Cat No. | New Cat No. | Pack Size |
|---|---|---|---|
| JAK2-Q Real-Time PCR Kit | G2M802621 | G610051-1 | 50 Tests |
Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.
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