Human Leukocyte Antigen B27 (HLA-B27) is a genetic biomarker strongly associated with a group of autoimmune and inflammatory disorders, particularly Ankylosing Spondylitis (AS), Psoriatic Arthritis, Reactive Arthritis, and inflammatory bowel disease-related arthritis. HLA-B27 positivity is observed in approximately 90% of patients with Ankylosing Spondylitis, making its detection an important tool in supporting early diagnosis and clinical assessment of autoimmune conditions. Accurate identification of the HLA-B27 allele can aid clinicians in evaluating patients presenting with chronic joint pain, spinal stiffness, inflammation, and related symptoms.
To address this important clinical need, Genes2Me introduces the HLA B27-Q Real-Time PCR Kit — an advanced in vitro diagnostic solution designed for the rapid and accurate detection of the HLA-B27 allele in human serum or plasma samples. Built on highly sensitive Real-Time PCR technology, the assay enables reliable molecular detection to support autoimmune disease diagnosis and patient management.
The HLA B27-Q Kit utilises a multiplex Real-Time PCR system incorporating highly specific primers and fluorescent probes targeting HLA-B27 sequences. Fluorescent reporter dyes enable simultaneous detection of HLA-B27-specific DNA and an internal control within separate detection channels, ensuring robust assay validation and minimising the risk of false-negative results. The optimised assay design delivers precise amplification and reliable performance across a broad range of clinical samples.
HLA-B27 testing is widely used as a supportive diagnostic marker in patients with suspected autoimmune and inflammatory disorders. Early and accurate detection of the HLA-B27 allele can help clinicians differentiate inflammatory arthritic diseases from other musculoskeletal conditions and support timely therapeutic intervention. With analytical sensitivity and specificity of 100%, clinical sensitivity and specificity of 98%, and a limit of detection of 800 copies/ml, the HLA B27-Q Kit provides laboratories with dependable molecular diagnostic performance.
Specifically detects HLA-B27 alleles associated with autoimmune and inflammatory disorders.
Simultaneous detection of target DNA and internal control for enhanced assay reliability.
100% analytical sensitivity and specificity with clinical sensitivity and specificity of 98%.
Easy reaction setup and rapid amplification protocol for efficient laboratory testing.
Validated on leading Real-Time PCR platforms including Thermo Fisher QuantStudio™ 5, Bio-Rad CFX96, Roche LightCycler® 480, and Genes2Me RapiCycler 96.
Excellent precision with repeatability and reproducibility CV < 3%.
With the HLA B27-Q Real-Time PCR Kit, laboratories can achieve highly sensitive, reproducible, and clinically meaningful detection of HLA-B27 alleles. By supporting early diagnosis and improved clinical evaluation of autoimmune diseases, the kit helps clinicians make informed decisions for patient care and disease management. Empower your laboratory with the HLA B27-Q Real-Time PCR Kit — because timely molecular insights can make a lasting difference in patient outcomes.
HLA B27-Q Real Time PCR specific amplification plot of Treponema pallidum Positive Control (HEX Channel) along with Internal Control (Cy5 Channel)
| Commercial Name | Old Cat No. | New Cat No. | Pack Size |
|---|---|---|---|
| HLA B27-Q Real-Time PCR Kit | G610046-1 | 50 Tests |
Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.
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