Wound Panel Real-Time PCR Kit

Qualitative Detection of Wound Infection-Causing Pathogens by Real-Time PCR

The Wound Panel Real-Time PCR Kit enables qualitative detection of major wound infection-causing pathogens from skin and soft tissue wound swab samples using real-time PCR. It supports rapid and reliable identification of multiple microbial agents associated with chronic wounds, facilitating comprehensive pathogen profiling in research settings.

At a Glance

Target: Chronic wound infection-causing pathogens
Technology: Real-Time PCR (multiplex, probe-based)
Sample Type: Skin and soft tissue wound swab samples
Detection Type: Qualitative
Application: Wound pathogen detection (Research Use Only)

Product Overview

The Wound Panel Real-Time PCR Kit is a multiplex molecular assay designed to detect and differentiate 25 major pathogens associated with chronic wound infections.

The assay utilizes pathogen-specific primers and fluorescent probes for simultaneous detection across multiple targets. Internal Controls are included to monitor assay performance and amplification efficiency.

How the Assay Works

Sample Collection & Nucleic Acid Extraction: DNA is extracted from wound swab samples using validated extraction methods.
Real-Time PCR Amplification: Extracted DNA is amplified using pathogen-specific primers and probes.
Fluorescent Detection: amplification signals are detected in real-time across fluorescence channels.
Result Interpretation: Ct value ≤ 35 is considered positive.

Key Features & Benefits

Multiplex detection of 25 wound-associated pathogens
Simultaneous detection of bacterial and fungal targets
Probe-based chemistry for high specificity
Internal control for assay validation
Rapid and reliable detection workflow

Why Wound Pathogen Testing Matters

Chronic wounds are a growing global health concern and are often associated with polymicrobial infections that complicate treatment and healing. Molecular detection using Real-Time PCR enables:

Rapid identification of causative pathogens
Detection of multiple organisms in a single test
Improved understanding of polymicrobial infections
Support for targeted therapeutic strategies in research

Sample Type & Reporting Output

Sample Types

Skin and soft tissue wound swab samples

Reporting Output

Qualitative detection (Positive/Negative for specific pathogens)

Targets Detected

Bacterial Targets

Staphylococcus aureus
Acinetobacter baumannii
Escherichia coli
Pseudomonas aeruginosa
Klebsiella pneumoniae
Staphylococcus epidermidis
Staphylococcus haemolyticus
Enterobacter aerogenes

Bacterial Targets

Enterococcus faecium
Listeria monocytogenes
Enterobacter cloacae
Streptococcus spp.
Enterococcus faecalis
Streptococcus pneumoniae
Stenotrophomonas maltophilia
Proteus mirabilis

Fungal Targets

Aspergillus fumigatus
Candida albicans
Candida parapsilosis
Candida tropicalis
Candida krusei
Fusarium spp.

Workflow Advantage

Multiplex assay enables simultaneous detection of 25 wound-associated pathogens across six reaction tubes

Pre-formulated primer and probe mixes simplify assay setup and reduce manual preparation steps

Internal control included within the assay design ensures reliable monitoring of amplification efficiency

Closed-tube real-time PCR workflow minimizes risk of contamination

Compatible with open real-time PCR platforms for flexible laboratory use

Storage and Stability

Shelf life: 12 months from date of manufacture
Storage: -20°C (shipped at -30°C to -10°C)
Avoid repeated freeze-thaw cycles (>6 times)
Protect from direct sunlight

Applications & Use Scenarios

The kit is intended for use in research laboratories for:

Detection of wound infection-causing pathogens
Polymicrobial infection profiling
Multiplex pathogen screening
Research on chronic wound microbiology

Ordering Information

Commercial Name	Old Cat No.	New Cat No.	Pack Size
Wound Panel Real Time PCR Kit	G2MBR4-0677-25T		25 Tests

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Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.