VZV-Q Real-Time PCR Kit

Quantitative Detection of Varicella-Zoster Virus (VZV) by Real-Time PCR

The VZV-Q Real-Time PCR Kit is an in vitro diagnostic molecular assay designed for sensitive and specific quantitative detection of Varicella-zoster virus (VZV) specific DNA in clinical samples using real-time PCR technology. It supports accurate detection of VZV infection for clinical diagnosis and laboratory applications.

At a Glance

Target: Varicella-zoster virus (VZV) DNA
Technology: Real-Time PCR (TaqMan probe-based chemistry)
Sample Type: Vesicular fluid, lesion swabs
Detection Type: Qualitative
Application: Detection of VZV infection

Product Overview

The VZV-Q Real-Time PCR Kit is based on TaqMan probe chemistry for the amplification and detection of VZV-specific DNA sequences. Fluorescent probes labelled with reporter and quencher dyes enable real-time monitoring of amplification.

The assay allows simultaneous detection of VZV DNA and an internal control (RNase P) using distinct fluorescence channels, ensuring assay reliability and validation of extraction and amplification steps.

Detection is performed across fluorescence channels and a positive control is included to verify proper assay setup, reagent integrity and amplification conditions.

How the Assay Works

The assay follows a standardized molecular workflow:

Sample Collection & Nucleic Acid Extraction: DNA is extracted from vesicular fluid or lesion swab samples using recommended extraction kits.
Real-Time PCR Amplification: Extracted DNA is amplified using VZV-specific primers in a real-time PCR system.
Fluorescent Detection: Amplification signals are detected in real time.
Quantification & Interpretation: Detection is based on Ct values and amplification curves for accurate result interpretation.

Key Features & Benefits

Quantitative detection of VZV DNA
TaqMan probe-based chemistry for high specificity
Multiplex detection (target + internal control)
Internal control ensures assay validation
Positive control for performance verification
Sensitive detection from lesion-based samples
Suitable for clinical diagnostic workflows

Why VZV Detection Matters

Varicella-zoster virus (VZV) is a human alpha herpesvirus responsible for both varicella (chickenpox) and herpes zoster (shingles).

• Primary infection causes varicella, characterized by fever and vesicular rash
• The virus remains latent in sensory nerve ganglia
• Reactivation leads to herpes zoster, often associated with age and immunosuppression

PCR is the most sensitive method for confirming VZV infection and can detect the virus in skin lesions such as vesicles, scabs, and maculopapular lesions. Compared to viral culture and DFA, PCR provides faster and more sensitive detection.

Targets Detected

VZV Target

Detection of Varicella-zoster virus DNA

Internal Control

RNase P (endogenous internal control)
Monitors nucleic acid extraction and amplification efficiency

Sample Type & Reporting Output

Sample Types

Vesicular fluid
Lesion swabs

Reporting Output

Quantitative detection of VZV DNA

Analytical Performance

Ct Cut-off Value:

≤ 35

Positive Control (VZV):

Ct value of 18 ± 2

Negative Control (NTC):

No amplification (Ct = 0)

Workflow advantage

Single-reaction setup with defined reagent volumes ensures consistency across runs

Multiplex detection of VZV target and internal control in a single reaction

Internal control (RNase P) integrated within assay design for validation

Pre-formulated reagents simplify assay setup

Closed-tube system minimizes contamination risk

Compatible with standard real-time PCR instruments

Storage and Stability

Shelf life: 12 months from date of manufacture
Storage temperature: -30°C to -10°C
Stable at -20°C until expiry
Avoid repeated freeze-thaw cycles (>6 times)
Protect from direct sunlight

Applications & Use Scenarios

The kit is intended for use in clinical laboratories for:

Detection of Varicella-zoster virus infection
Diagnosis of varicella and herpes zoster
Molecular testing of lesion-based samples
Routine diagnostic workflows

Ordering Information

Commercial Name	Old Cat No.	New Cat No.	Pack Size
VZV-Q Real-Time PCR Kit	G2M705821		50 Tests

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