UTI-Q Real Time PCR Kit

Qualitative Detection of Urinary Tract Infection Pathogens by Real-Time PCR

The UTI-Q Real-Time PCR Kit enables qualitative detection of multiple urinary tract infection-associated pathogens in human urine samples using multiplex real-time PCR. It supports rapid identification of infectious agents for research applications and pathogen profiling.

At a Glance

Target: Urinary tract infection-causing pathogens
Technology: Real-Time PCR (multiplex, probe-based)
Sample Type: Human urine
Detection Type: Qualitative
Application: Research use for UTI pathogen detection

Product Overview

The UTI-Q Real-Time PCR Kit is a multiplex molecular assay designed for the detection of 19 major pathogens associated with urinary tract infections.

The assay utilizes pathogen-specific primers and fluorescent probes labelled with multiple dyes for simultaneous detection. An exogenous internal control is incorporated to monitor extraction efficiency and PCR performance.

How the Assay Works

Sample Collection & Nucleic Acid Extraction: DNA is extracted from urine samples collected using a clean-catch midstream method. Exogenous internal control is added during extraction.
Real-Time PCR Amplification: Extracted DNA is amplified using pathogen-specific primers and probes in multiplex reactions.
Fluorescent Detection: Amplification signals are detected across fluorescence channels.
Result Interpretation: Ct value ≤ 30 is considered positive.

Key Features & Benefits

Multiplex detection of 19 UTI-associated pathogens
Simultaneous detection across bacterial and fungal targets
Probe-based chemistry for enhanced specificity
Exogenous internal control for assay validation
Rapid and reliable detection workflow

Why UTI Pathogen Testing Matters

Urinary tract infections are among the most common bacterial infections and can lead to complications if not properly managed. Molecular detection using Real-Time PCR enables:

Rapid identification of causative pathogens
High sensitivity and specificity compared to conventional methods
Detection of multiple organisms in a single test
Support for targeted antimicrobial research and studies

Sample Type & Reporting Output

Sample Types

Urine samples (clean-catch midstream)

Reporting Output

Qualitative detection (Positive/Negative for specific pathogens)

Targets Detected

Bacterial Targets

Escherichia coli
Klebsiella pneumoniae
Staphylococcus aureus
Staphylococcus spp.
Staphylococcus epidermidis
Enterococcus faecalis
Salmonella enterica

Bacterial Targets

Enterococcus faecium
Pseudomonas aeruginosa
Acinetobacter baumannii
Stenotrophomonas maltophilia
Haemophilus influenzae type B
Streptococcus pneumoniae
Listeria monocytogenes

Fungal Targets

Candida albicans
Candida glabrata
Candida tropicalis
Aspergillus spp.

Workflow Advantage

Multiplex detection across seven ready-mix tubes enables broad pathogen coverage in a single workflow

Pre-formulated ready mixes reduce manual preparation steps and improve consistency

Integration of exogenous internal control ensures reliable extraction and amplification monitoring

Closed-tube real-time PCR system minimizes contamination risk during processing

Compatible with standard open real-time PCR platforms for flexible laboratory implementation

Storage and Stability

Shelf life: 12 months from date of manufacture
Storage: -20°C (shipped at -30°C to -10°C)
Avoid repeated freeze-thaw cycles (>6 times)
Protect from direct sunlight

Applications & Use Scenarios

The kit is intended for use in research laboratories for:

UTI pathogen detection and profiling
Multiplex pathogen screening
Microbiological and molecular research studies
Support for antimicrobial research and epidemiological studies

Ordering Information

Commercial Name	Old Cat No.	New Cat No.	Pack Size
UTI-Q Real Time PCR Kit	G2MBR4-0678		25 Tests

Product Enquiry Form

Other Products

Rapi-Q

Rapid and Compact Point-of-care RT-PCR Testing Solution.

RAPiCycler-96

Real time PCR system to process 96 samples.

Rapi-X16

Compact Nucleic Acid Extraction System Ideal for Point-of-Care Testing

Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.