Sepsis-Q Real Time PCR Kit

Qualitative Detection of Sepsis-Causing Pathogens by Real-Time PCR

The Sepsis-Q Real-Time PCR Kit is designed for the detection of sepsis-related pathogen DNA in human whole blood and positive blood culture samples using multiplex real-time PCR technology. The assay enables simultaneous detection of multiple pathogens associated with bloodstream infections.

At a Glance

Product Overview

The assay utilizes multiplex real-time PCR for the amplification of pathogen-specific DNA sequences. The kit consists of a qRT-PCR Master Mix, and 8 primer-probe mix tubes, each designed to detect specific pathogens using distinct fluorescence channels, enabling simultaneous identification of multiple pathogens along with internal control validation.

Sepsis-Q Comprehensive

How the Assay Works

The assay follows a standardized molecular workflow:

  • Sample Collection & Nucleic Acid Extraction: DNA is extracted from whole blood or positive blood culture.
  • Reaction Setup : Sample DNA is added to reaction mix with pathogen-specific primer-probe sets
  • PCR Amplification: Target DNA sequences are amplified
  • Fluorescent Detection: Amplification signals are detected using multiple fluorescence channels
  • Result Interpretation: Based on pathogen-specific amplification patterns

Targets Detected

Bacterial Targets
  • Staphylococcus aureus
  • Acinetobacter baumannii
  • Escherichia coli
  • Pseudomonas aeruginosa
  • Klebsiella pneumoniae
  • CoNS spp. (Staphylococcus epidermidis, Staphylococcus haemolyticus)
  • Enterococcus faecium
  • Listeria monocytogenes
Bacterial Targets
  • Enterobacter cloacae
  • Streptococcus spp. (S. pyogenes, S. agalactiae)
  • Enterococcus faecalis
  • Streptococcus pneumoniae
  • Stenotrophomonas maltophilia
  • Enterococcus gallinarum
  • Serratia marcescens
  • Campylobacter spp.
  • Campylobacter spp.
Fungal Targets
  • Aspergillus fumigatus
  • Candida albicans
  • Non-albicans Candida spp. (C. parapsilosis, C. tropicalis, C. krusei, C. auris, C. glabrata)
  • Fusarium spp.

Key Features & Benefits

  • Detection of 32 major sepsis-causing pathogens
  • Multiplex assay design for comprehensive coverage
  • Probe-based detection for specificity
  • Integrated internal control system
  • Rapid pathogen identification

Why Sepsis Detection Matters

Sepsis is a life-threatening condition caused by systemic infection and can rapidly progress to organ failure if not diagnosed early. Rapid and accurate identification of causative pathogens is critical for timely treatment and improved patient outcomes.

Sample Type & Reporting Output

Sample Types
  • Whole blood
  • Positive blood culture
Reporting Output
  • Qualitative detection of specific sepsis-causing pathogens

Analytical Performance

Analytical Sensitivity:
100%
Analytical Specificity:
100%
Trueness (Bias):
99%
Accuracy:
99%
Limit of Detection (LoD):
100 copies/ml
Measuring Range:
1 – 10⁹ copies/reaction
Cut-off:
Ct ≤ 35
Cross-reactivity:
Not observed (Chlamydia pneumoniae, Mycoplasma pneumoniae, Bordetella pertussis, Legionella pneumophila)
Clinical Sensitivity:
98%
Clinical Specificity:
99%

Workflow Advantage

Simultaneous detection of 32 pathogens in a multiplex format
Reduced number of reactions through tube-based panel design
Faster turnaround compared to conventional culture methods
Enables high-throughput screening in clinical laboratories

Designed for Laboratory Efficiency

The assay is optimized for streamlined workflow:

  • Pre-formulated primer-probe mixes (8-tube system)
  • Standardized reaction setup
  • Compatible with multiple real-time PCR platforms
  • Closed-tube detection reduces contamination risk

Storage and Stability

  • Shelf life: 12 months from date of manufacture
  • Storage: -20°C (shipped at -30°C to -10°C)
  • Avoid repeated freeze-thaw cycles (>6 times)
  • Protect from direct sunlight

Applications & Use Scenarios

The kit is intended for use in clinical and research laboratories for:

  • Detection of sepsis-causing pathogens
  • Bloodstream infection screening
  • Rapid infectious disease diagnostics
  • Clinical microbiology workflows
  • Sepsis-related research

Ordering Information

Commercial Name Old Cat No. New Cat No. Pack Size
Sepsis-Q Real-Time PCR Kit G2M804026 25 Tests

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