Rota-Q Real Time PCR Kit for Rotavirus

Qualitative Detection of Rotavirus by Real-Time PCR

The Rota-Q Real-Time PCR Kit enables qualitative detection of Rotavirus RNA in clinical samples using real-time PCR. It supports rapid and reliable identification of Rotavirus infections for effective clinical decision-making.

At a Glance

Target: Rotavirus
Technology: Real-Time PCR (multiplex, probe-based)
Sample Type: Stool samples
Detection Type: Qualitative
Application: Rotavirus infection detection

Product Overview

The Rota-Q Real-Time PCR Kit is a molecular diagnostic assay designed for the detection of Rotavirus RNA from clinical samples.

The assay utilizes pathogen-specific primers and fluorescent probes for accurate amplification and detection of Rotavirus RNA. An internal control is included to monitor amplification efficiency and ensure assay reliability.

How the Assay Works

Sample Collection & Nucleic Acid Extraction: RNA is extracted from stool samples using validated extraction methods.
Real-Time PCR Amplification: Extracted RNA is amplified using Rotavirus-specific primers and probes.
Fluorescent Detection: Amplification signals are detected in real-time, enabling identification of Rotavirus RNA along with internal control validation.
Result Interpretation: Ct value ≤ 35 is considered positive.

Key Features & Benefits

Specific detection of Rotavirus RNA
Probe-based chemistry for high specificity
Internal control for assay validation
Rapid and reliable results
Suitable for routine clinical laboratory workflows

Why Rotavirus Testing Matters

Rotavirus is a leading cause of severe diarrhoea in infants and young children worldwide. Molecular detection using Real-Time PCR enables:

Rapid identification of Rotavirus infections
High sensitivity and specificity compared to conventional methods
Early diagnosis to support timely clinical management
Improved patient outcomes, especially in paediatric populations

Sample Type & Reporting Output

Sample Types

Stool samples

Reporting Output

Qualitative detection (Positive/Negative for Rotavirus)

Workflow Advantage

Pre-formulated reagents simplify assay setup

Single-target detection with internal control reduces complexity

Minimal handling steps improve workflow efficiency

Closed-tube system minimizes contamination risk

Compatible with standard real-time PCR instruments

Storage and Stability

Shelf life: 12 months from date of manufacture
Storage: -20°C (shipped at -30°C to -10°C)
Avoid repeated freeze-thaw cycles (>6 times)
Protect from direct sunlight

Applications & Use Scenarios

The kit is intended for use in clinical laboratories for:

Detection of Rotavirus infections
Routine diagnostic workflows
Support for clinical decision-making

Ordering Information

Commercial Name	Old Cat No.	New Cat No.	Pack Size
Rota-Q Real-Time PCR Kit	G2M801621		50 Tests

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