Nipah-Q Real Time PCR Kit

Qualitative Detection of Nipah Virus by Real-Time PCR

The Nipah-Q Real-Time PCR Kit enables qualitative detection of Nipah virus RNA in human respiratory samples using real-time RT-PCR. It supports rapid and reliable identification of NiV infection to aid in timely diagnosis and outbreak management.

At a Glance

Target: Nipah Virus (NiV)
Technology: Real-Time RT-PCR (probe-based)
Sample Type: Oropharyngeal and Nasopharyngeal swabs
Detection Type: Qualitative
Application: Nipah virus detection in clinical samples

Product Overview

The Nipah-Q Real-Time PCR Kit is a probe-based molecular assay designed for the detection of Nipah virus RNA targeting the Poly gene region. The assay utilizes specific primers and fluorescent probes for virus detection along with an internal control to validate extraction and amplification efficiency.

How the Assay Works

Sample Collection & RNA Extraction: RNA is extracted from oropharyngeal and nasopharyngeal swab samples using validated extraction methods.
Real-Time PCR Amplification: Extracted RNA undergoes reverse transcription followed by amplification using NiV-specific primers.
Fluorescent Detection: Amplification signals are detected in amplification signals are detected in real-time across fluorescence channels.
Result Interpretation: Ct value ≤ 35 is considered positive.

Key Features & Benefits

Single-tube RT-PCR assay for simplified workflow
Specific detection of Nipah virus RNA
Integrated internal control for assay validation
Rapid and reliable detection
Suitable for outbreak and surveillance applications

Why Nipah Virus Detection Matters

Nipah virus is a highly pathogenic zoonotic virus associated with severe respiratory illness and fatal encephalitis. Molecular detection using Real-Time RT-PCR enables:

Rapid identification of infection
Early detection during outbreaks
High sensitivity and specificity
Support for infection control and public health response

Sample Type & Reporting Output

Sample Types

Oropharyngeal swabs
Nasopharyngeal swabs

Reporting Output

Qualitative detection (Positive/Negative for Nipah virus)

Workflow Advantage

Single-tube RT-PCR assay reduces handling steps and simplifies workflow

Pre-optimized reagents ensure consistent amplification performance

Integrated internal control validates both extraction and amplification steps

Closed-tube detection minimizes contamination risk

Compatible with standard real-time PCR platforms for routine laboratory use

Analytical Performance

The assay demonstrates reliable multiplex detection performance:

Limit of Detection (LOD):

5 copies/reaction

Analytical Specificity:

No interference observed with tested substances (nasal spray, antibiotics, human genomic DNA)

Cross-Reactivity:

No cross-reactivity observed with Chlamydia pneumoniae, CMV, and VZV

Clinical Sensitivity:

98%

Clinical Specificity:

99%

Storage and Stability

Shelf life: 12 months from date of manufacture
Storage: -20°C (shipped at -30°C to -10°C)
Avoid repeated freeze-thaw cycles (>6 times)
Protect from direct sunlight

Applications & Use Scenarios

The kit is intended for use in clinical laboratories for:

Detection of Nipah virus infection
Outbreak surveillance and monitoring
Respiratory pathogen testing workflows
Support for public health response

Ordering Information

Commercial Name	Old Cat No.	New Cat No.	Pack Size
Nipah-Q Real-Time PCR Kit	G2M705921		50 Tests

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Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.