MCP-Q Comprehensive Real Time PCR Kit

Qualitative Detection of Meningitis-Causing Pathogens by Real-Time PCR

The MCP-Q Comprehensive Real-Time PCR Kit enables qualitative detection of meningitis-associated viral and bacterial pathogens in human clinical samples using real-time PCR. It supports rapid and reliable identification to aid timely diagnosis and clinical decision-making.

At a Glance

Target: Meningitis-causing viral and bacterial pathogens
Technology: Real-Time PCR (probe-based multiplex assay)
Sample Type: CSF, plasma, whole blood
Detection Type: Qualitative
Application: Meningitis pathogen detection

Product Overview

The MCP-Q Comprehensive Real-Time PCR Kit is a multiplex molecular assay designed for the detection of nucleic acids from meningitis-causing pathogens in human clinical samples.

The assay uses pathogen-specific primers and fluorescent probes, each tagged with distinct fluorophores, enabling simultaneous detection of multiple targets. The kit includes an Exogenous Internal Control (EIC) to monitor extraction and amplification efficiency.

How the Assay Works

Sample Collection & Nucleic Acid Extraction: Nucleic acid is extracted from CSF, plasma, or whole blood samples. Exogenous Internal Control is added during extraction.
Real-Time PCR Amplification: - Extracted nucleic acid is amplified using pathogen-specific primers in a real-time PCR system.
Fluorescent Detection: Amplification signals are detected using multiple fluorescence channels.
Result Interpretation: Ct values ≤ 40 are considered positive for target detection.

Key Features & Benefits

Multiplex detection of multiple meningitis pathogens
High sensitivity and specificity
Probe-based detection for accurate identification
Inclusion of Exogenous Internal Control
Simultaneous viral and bacterial detection
Rapid turnaround time compared to conventional methods

Why Meningitis Testing Matters

Meningitis is an inflammation of the meninges caused by viral, bacterial, fungal, or parasitic infections. It can progress rapidly and may lead to severe complications if not diagnosed early. Molecular detection using Real-Time PCR enables:

Rapid identification of causative pathogens
High sensitivity for low pathogen load
Simultaneous detection of multiple pathogens
Faster clinical decision-making compared to conventional methods

Targets Detected

Viral Targets

Herpes Simplex Virus 1 (HSV1)
Herpes Simplex Virus 2 (HSV2)
Varicella-Zoster Virus (VZV)
Human Herpesvirus 6, 7 (HHV6, HHV7)
Parvovirus B19
Enterovirus
Parechovirus

Bacterial Targets

Haemophilus influenzae
Neisseria meningitidis
Streptococcus pneumoniae

Exogenous Internal Control (EIC):

Monitors nucleic acid extraction and amplification efficiency

Sample Type & Reporting Output

Sample Types

Cerebrospinal Fluid (CSF)
Plasma
Whole blood

Reporting Output

Qualitative detection (Positive/Negative for specific pathogens)

Workflow Advantage

Pre-formulated ready mixes reduce preparation steps

Multiplex detection across four tubes

Minimal handling reduces contamination risk

Compatible with standard real-time PCR platforms

Streamlined workflow for clinical laboratories

Designed for Laboratory Efficiency

Ready-to-use reaction mixes
Reduced pipetting steps
Integrated internal control system
Compatible with commonly used PCR instruments

Storage and Stability

Shelf life: 12 months from date of manufacture
Storage: -20°C (shipped at -30°C to -10°C)
Avoid repeated freeze-thaw cycles (>6 times)
Protect from direct sunlight

Applications & Use Scenarios

The kit is intended for use in clinical laboratories for:

Detection of meningitis-causing pathogens
Multiplex pathogen identification
Rapid diagnostic workflows
Support for clinical decision-making

Ordering Information

Commercial Name	Old Cat No.	New Cat No.	Pack Size
MCP-Q Comprehensive Real-Time PCR Kit for Meningitis	G2M803821-25T		25 Tests

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