B19-Q Real-Time PCR Kit

Qualitative Detection of Human Parvovirus (B19) DNA by Real-Time PCR

The B19-Q Real-Time PCR Kit enables qualitative detection of Human Parvovirus B19 DNA in human clinical samples using real-time PCR technology. It provides a reliable molecular method for identifying Parvovirus infection in clinical laboratory settings.

At a Glance

Target: Human Parvovirus B19
Technology: Real-Time PCR (probe-based)
Sample Type: Whole blood, plasma, serum
Detection Type: Qualitative
Application: Detection of Parvovirus B19 infection

Product Overview

The B19-Q Real-Time PCR Kit is an in vitro diagnostic assay designed for the detection of Parvovirus B19 DNA using specific primers and fluorescent probes.

The assay enables simultaneous detection of:

Parvovirus B19 DNA
Internal Control

The inclusion of an Internal Control ensures validation of extraction efficiency and amplification performance, reducing the risk of false-negative results.

How the Assay Works

Sample Collection & DNA Extraction: DNA is extracted from whole blood, plasma, or serum using validated extraction methods.
Real-Time RT-PCR Amplification: Target DNA is amplified using specific primers and probes for Parvovirus B19.
Fluorescent Detection: Amplification signals are detected in real time using probe-based chemistry for each target.
Result Interpretation: Ct value ≤ 35 is considered positive.

Key Features & Benefits

Specific detection of Parvovirus B19 DNA
Probe-based chemistry for high specificity
Internal Control for assay validation
Reliable qualitative results
Suitable for routine clinical laboratory workflows

Why Parvovirus B19 Testing Matters

Parvovirus B19 is a highly contagious virus commonly associated with childhood infections such as fifth disease (slapped-cheek disease), but it can also affect adults.

Key clinical considerations include:

Causes erythema infectiosum in children
Can lead to joint pain and swelling in adults
May cause severe anaemia in certain patient groups
Often asymptomatic but clinically significant in immunocompromised individuals

Molecular detection using Real-Time PCR offers:

High sensitivity and specificity
Early and accurate detection
Reliable identification even in low viral load samples

Sample Type & Reporting Output

Sample Types

Whole blood, plasma, serum

Reporting Output

Qualitative detection (Positive / Negative)

Workflow Advantage

Single-tube assay simplifies workflow and reduces handling steps

Pre-optimized reagents ensure consistent assay performance

Internal control integrated within assay design for result validation

Closed-tube system minimizes contamination risk

Compatible with standard real-time PCR instruments

Analytical Performance

Analytical Sensitivity:

100%

Analytical Specificity:

100%

Trueness (Bias):

98%

Accuracy:

99%

Limit of Detection (LOD):

100 copies/ml

Measuring Range:

10 – 10⁹ copies/reaction

Clinical Sensitivity:

99%

Clinical Specificity:

99.1%

Storage and Stability

Shelf life: 12 months from date of manufacture
Storage: -20°C (shipped at -30°C to -10°C)
Perform assay at: 15°C to 30°C
Protect from direct sunlight

Applications & Use Scenarios

The kit is intended for use in clinical laboratories for:

Detection of Parvovirus B19 infection
Routine molecular diagnostic workflows
Screening of suspected viral infections
Supporting clinical diagnosis and patient management

Ordering Information

Commercial Name	Old Cat No.	New Cat No.	Pack Size
B19-Q Real-Time PCR Kit	G2M706521		50 Tests

Product Enquiry Form

Other Products

Rapi-Q

Rapid and Compact Point-of-care RT-PCR Testing Solution.

RAPiCycler-96

Real time PCR system to process 96 samples.

Rapi-X16

Compact Nucleic Acid Extraction System Ideal for Point-of-Care Testing

Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.