GCP-Q Comprehensive Real-Time PCR Kit

Qualitative Detection of Gastrointestinal Infection-Causing Pathogens by Real-Time PCR

The GCP-Q Comprehensive Real-Time PCR Kit enables qualitative detection of major gastrointestinal pathogens in human clinical samples using real-time PCR. It supports rapid and reliable identification of multiple infectious agents to aid diagnosis and clinical decision-making.

At a Glance

Target: Gastrointestinal infection-causing pathogens
Technology: Real-Time PCR (multiplex, probe-based)
Sample Type: Stool, gastric biopsy specimens
Detection Type: Qualitative
Application: Gastrointestinal pathogen detection

Product Overview

The GCP-Q Comprehensive Real-Time PCR Kit is a multiplex molecular assay designed to detect and differentiate 14 major gastrointestinal pathogens from human samples. The assay utilizes pathogen-specific primers and fluorescent probes for simultaneous detection across multiple targets. An Exogenous Internal Control (EIC) is included to monitor PCR inhibition and overall assay performance.

How the Assay Works

The assay follows a standardized molecular workflow:

Sample Collection & Nucleic Acid Extraction: DNA/RNA is extracted from stool or gastric biopsy samples using validated extraction methods.
Real-Time PCR Amplification: Extracted nucleic acid is amplified using pathogen-specific primers and probes.
Fluorescent Detection: Amplification signals are detected across multiple fluorescence channels.
Result Interpretation: Ct value ≤ 30 is considered positive.

Key Features & Benefits

Multiplex detection of 14 gastrointestinal pathogens
Simultaneous detection of viral, bacterial, and parasitic targets
Probe-based chemistry for specificity
Exogenous Internal Control for assay validation
Rapid and reliable results

Why Gastrointestinal Pathogen Testing Matters

Gastrointestinal infections are caused by a wide range of viral, bacterial, and parasitic pathogens and are a major cause of morbidity worldwide, especially in children.

Molecular detection using Real-Time PCR enables:

Rapid identification of causative pathogens
Detection of multiple organisms in a single test
Improved diagnostic accuracy
Support for timely clinical management

Targets Detected

Viral Targets

Astrovirus
Rotavirus
Norovirus Genotype I, II
Adenovirus

Bacterial Targets

Clostridium difficile
Campylobacter spp.
Salmonella spp
Enteroinvasive E. coli (EIEC)/Shigella spp.

Exogenous Internal Control (EIC):

Monitors PCR inhibition and overall assay performance

Sample Type & Reporting Output

Sample Types

Stool samples
Gastric biopsy specimens

Reporting Output

Qualitative detection (Positive/Negative for specific pathogens)

Workflow Advantage

Pre-formulated ready mixes simplify assay setup

Multiplex detection across five tubes

Reduced handling and contamination risk

Compatible with standard real-time PCR systems

Efficient workflow for clinical laboratories

Designed for Laboratory Efficiency

The assay is optimized for streamlined workflow:

Ready-to-use reagents
Minimal pipetting steps
Integrated internal control system
Compatible with widely used PCR platforms

Storage and Stability

Shelf life: 12 months from date of manufacture
Storage temperature: -30°C to -10°C
Stable at -20°C until expiry
Avoid repeated freeze-thaw cycles (>6 times)
Protect from direct sunlight

Applications & Use Scenarios

The kit is intended for use in clinical laboratories for:

Detection of gastrointestinal infection-causing pathogens
Multiplex pathogen screening
Routine diagnostic workflows
Support for clinical decision-making

Ordering Information

Commercial Name	Old Cat No.	New Cat No.	Pack Size
GCP-Q Comprehensive Real-Time PCR Kit	G2M803621-25T		25 Tests

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Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.