EEC-Q Real-Time PCR Kit

Qualitative Detection of Enteroviruses by Real-Time PCR

The EEC-Q Real-Time PCR Kit enables qualitative detection of major enteroviruses associated with Hand-Foot-Mouth Disease (HFMD) using real-time RT-PCR. It allows simultaneous identification of multiple viral targets in a single reaction for efficient and reliable detection.

At a Glance

Target: Enterovirus (Universal), Enterovirus 71 (EV71), Coxsackievirus A16 (CoxA16)
Technology: Real-Time RT-PCR (single-tube multiplex, probe-based)
Sample Type: Pharyngeal swabs (posterior pharyngeal wall and tonsils)
Detection Type: Qualitative
Application: Detection of Hand-Foot-Mouth Disease (HFMD) causing viruses

Product Overview

The EEC-Q Real-Time PCR Kit is a single-tube multiplex molecular assay designed for the detection and differentiation of three major enteroviruses from human clinical samples. The assay utilizes pathogen-specific primers and fluorescent probes for simultaneous detection of multiple viral RNA targets along with an internal control to ensure assay reliability.

How the Assay Works

Sample Collection & RNA Extraction: RNA is extracted from pharyngeal swab samples using validated extraction methods.
Real-Time RT-PCR Amplification: Extracted RNA is reverse transcribed and amplified using target-specific primers and probes.
Fluorescent Detection: Amplification signals are monitored in real time to detect specific viral RNA targets and internal control.
Result Interpretation: Ct value ≤35 is considered positive for target detection.

Key Features & Benefits

Single-tube multiplex assay for multiple viral targets
Simultaneous detection and differentiation of HFMD-causing viruses
Probe-based chemistry for high specificity and sensitivity
Internal control for assay validation
Rapid and reliable detection of viral RNA

Why Enterovirus Testing Matters

Hand-Foot-Mouth Disease (HFMD) is a highly contagious viral illness primarily affecting children under five years of age. Molecular detection using real-time RT-PCR enables:

Rapid identification of causative viral pathogens
Differentiation between Enterovirus, EV71, and CoxA16
Early detection of potentially severe infections
Support for timely clinical and epidemiological decision-making

Sample Type & Reporting Output

Sample Types

Posterior pharyngeal swabs
Tonsillar swabs

Reporting Output

Qualitative detection (Positive/Negative for BK virus)

Targets Detected

Viral Target

Enterovirus (Universal)
Enterovirus 71 (EV71)
Coxsackievirus A16 (CoxA16)

Internal Control

Monitors RNA extraction efficiency and PCR performance

Workflow Advantage

Single-tube multiplex assay reduces handling steps

Pre-formulated reagents simplify assay setup

Integrated internal control ensures assay reliability

Closed-tube system minimizes contamination risk

Compatible with standard real-time PCR instruments

Analytical Performance

Ct cut-off:

≤35 for positive detection

Positive control Ct:

≤30 for all targets

No amplification in NTC confirms absence of contamination

Internal control validates extraction and amplification efficiency

Storage and Stability

Shelf life: 12 months from date of manufacture
Storage: -20°C (shipped at -30°C to -10°C)
Avoid repeated freeze-thaw cycles (>6 times)
Protect from direct sunlight

Applications & Use Scenarios

The kit is intended for use in clinical laboratories for:

Detection of enteroviruses associated with HFMD
Multiplex viral screening
Routine diagnostic workflows
Epidemiological surveillance and outbreak monitoring

Ordering Information

Commercial Name	Old Cat No.	New Cat No.	Pack Size
EEC-Q Real-Time PCR Kit	G2M801321		50 Tests

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Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.