EBV-Q Real-Time PCR Kit

Qualitative Detection of Epstein-Barr Virus by Real-Time PCR

The EBV-Q Real-Time PCR Kit enables qualitative detection of Epstein-Barr Virus (EBV 1 & 2) in human clinical samples using real-time PCR. It supports rapid and reliable identification of EBV DNA to aid in clinical diagnosis and monitoring.

At a Glance

Target: Epstein-Barr Virus (EBV 1 & 2)
Technology: Real-Time PCR (probe-based)
Sample Type: Plasma, Serum, CSF
Detection Type: Qualitative
Application: EBV detection in clinical samples

Product Overview

The EBV-Q Real-Time PCR Kit is a probe-based molecular assay designed for the detection of Epstein-Barr Virus DNA in human samples. The assay utilizes specific primers and fluorescent probes for EBV detection, along with an internal control to ensure assay integrity and monitor amplification performance.

How the Assay Works

Sample Collection & Nucleic Acid Extraction: DNA is extracted from plasma, serum, or CSF samples using validated extraction methods.
Real-Time PCR Amplification: Extracted DNA is amplified using EBV-specific primers in a single-tube reaction.
Fluorescent Detection: Amplification signals are detected in amplification signals are detected in real-time across fluorescence channels.
Result Interpretation: Ct value ≤ 35 is considered positive.

Key Features & Benefits

Single-tube assay design for simplified workflow
Specific detection of EBV DNA using probe-based chemistry
Integrated internal control for assay validation
Rapid and reliable detection
High sensitivity and specificity

Why EBV Detection Matters

Epstein-Barr Virus is one of the most common human viruses and is associated with infectious mononucleosis and other clinical conditions. Molecular detection using Real-Time PCR enables:

Rapid and sensitive detection of EBV DNA
Early identification of infection and reactivation
Improved diagnostic accuracy compared to conventional methods
Support for monitoring in immunocompromised patients

Sample Type & Reporting Output

Sample Types

Plasma, Serum, CSF

Reporting Output

Qualitative detection (Positive/Negative for EBV)

Workflow Advantage

Single-tube assay reduces handling steps and simplifies workflow

Pre-optimized master mix ensures consistent amplification performance

Internal control integrated within the assay enables reliable result validation

Closed-tube detection minimizes contamination risk

Compatible with standard real-time PCR instruments for routine laboratory use

Analytical Performance

The assay demonstrates reliable multiplex detection performance:

Analytical Sensitivity:

100%

Trueness (Bias):

99%

Limit of Detection (LoD):

1100 copies/ml

Measuring Range:

1 – 10⁹ copies/reaction

Clinical Sensitivity:

96%

Clinical Specificity:

99%

No cross-reactivity observed with HSV-1, HSV-2, Cytomegalovirus, and HIV

Storage and Stability

Shelf life: 12 months from date of manufacture
Storage: -20°C (shipped at -30°C to -10°C)
Avoid repeated freeze-thaw cycles (>6 times)
Protect from direct sunlight

Applications & Use Scenarios

The kit is intended for use in clinical laboratories for:

Detection of Epstein-Barr Virus infection
Monitoring EBV-associated conditions
Routine molecular diagnostic workflows
Support for clinical decision-making

Ordering Information

Commercial Name	Old Cat No.	New Cat No.	Pack Size
EBV-Q Real-Time PCR Kit	G2M705621		50 Tests

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Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.