CMV-Q Real Time PCR Kit

Quantitative Detection of Cytomegalovirus (CMV) by Real-Time PCR

The CMV-Q Real-Time PCR Kit enables detection and quantification of Cytomegalovirus DNA in human blood samples using real-time PCR. It supports accurate viral load measurement for effective disease monitoring and clinical management.

At a Glance

Target: Cytomegalovirus (CMV)
Technology: Real-Time PCR (quantitative, multiplex, probe-based)
Sample Type: EDTA whole blood
Detection Type: Qualitative
Application: CMV viral load detection and monitoring

Product Overview

The CMV-Q Real-Time PCR Kit is a multiplex quantitative molecular assay designed for precise detection and measurement of CMV DNA in clinical samples. The assay utilizes CMV-specific primers and probes along with an internal control to ensure reliable amplification and result validation. The kit includes multiple quantification standards that enable generation of a standard curve for accurate viral load determination.

How the Assay Works

Sample Collection & DNA Extraction: DNA is extracted from EDTA whole blood samples using validated extraction methods.
Real-Time PCR Amplification: Extracted DNA is amplified using CMV-specific primers in a quantitative PCR reaction.
Quantification Using Standard Curve: - Quantification standards are used to generate a calibration curve for precise viral load estimation.
Result Interpretation: Ct value ≤35 is considered positive, and viral load is calculated using the standard curve.

Key Features & Benefits

Quantitative detection of CMV DNA
Multiplex assay with internal control
Includes multiple quantification standards for accurate viral load analysis
Probe-based chemistry for high specificity
Reliable and reproducible results

Why CMV Testing Matters

Cytomegalovirus (CMV) is a common herpesvirus that can cause severe disease in immunocompromised individuals and newborns. Molecular detection using real-time PCR enables:

Early and sensitive detection of CMV infection
Accurate viral load monitoring
Better management of transplant and immunocompromised patients
Timely therapeutic intervention

Sample Type & Reporting Output

Sample Types

EDTA whole blood

Reporting Output

Quantitative detection (Viral load measurement of CMV DNA)

Workflow Advantage

Pre-formulated reagents simplify assay setup

Integrated quantification standards enable accurate viral load estimation

Internal control ensures assay reliability

Closed-tube system minimizes contamination risk

Compatible with standard real-time PCR instruments

Analytical Performance

Analytical Sensitivity:

100%

Analytical Specificity:

100%

Accuracy:

99%

Limit of Detection (LOD):

50 copies/ml

Measuring Range:

10 – 10⁹ copies/reaction

Slope of calibration curve:

-3.31

Cross-reactivity:

No cross-reactivity observed (HIV, HCV, HBV, HSV)

Clinical Sensitivity:

99%

Clinical Specificity:

99%

Storage and Stability

Shelf life: 12 months from date of manufacture
Storage temperature: -30°C to -10°C
Stable at recommended storage conditions until expiry
Avoid repeated freeze-thaw cycles (>6 times)
Protect from direct sunlight

Applications & Use Scenarios

The kit is intended for use in clinical laboratories for:

CMV viral load monitoring
Infection detection in immunocompromised patients
Transplant patient management
Routine molecular diagnostic workflows

Ordering Information

Commercial Name	Old Cat No.	New Cat No.	Pack Size
CMV-Q Real-Time PCR Kit	G2MBR4-0075		50 Tests

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