BK-Q Real-Time PCR Kit

Qualitative Detection of BK Virus by Real-Time PCR

The BK-Q Real-Time PCR Kit enables qualitative detection of BK virus DNA in human clinical samples using real-time PCR. It supports rapid and reliable identification of BKV to aid in clinical diagnosis and monitoring.

At a Glance

Target: BK Virus (BKV)
Technology: Real-Time PCR (probe-based)
Sample Type: Blood, Serum, Plasma
Detection Type: Qualitative
Application: BK virus detection in clinical samples

Product Overview

The BK-Q Real-Time PCR Kit is a probe-based molecular assay designed for the detection of BK virus DNA in clinical samples. The assay utilizes specific primers and fluorescent probes targeting BK virus DNA, along with an internal control to ensure assay integrity and monitor amplification efficiency.

How the Assay Works

Sample Collection & Nucleic Acid Extraction: DNA is extracted from blood, serum, or plasma samples using validated extraction methods.
Real-Time PCR Amplification: Extracted DNA is amplified using BK virus-specific primers in a real-time PCR reaction.
Fluorescent Detection: Amplification signals are detected in amplification signals are detected in real-time across fluorescence channels.
Result Interpretation: Ct value ≤ 35 is considered positive.

Key Features & Benefits

Single-tube assay design for streamlined workflow
Specific detection of BK virus DNA using probe-based chemistry
Integrated internal control for assay validation
Rapid and reliable detection
High sensitivity and specificity

Why BK Virus Detection Matters

BK virus is a common human polyomavirus that establishes latent infection and can reactivate, especially in immunocompromised individuals. Molecular detection using Real-Time PCR enables:

Sensitive detection of viral DNA
Early identification of viral reactivation
Improved monitoring in transplant and immunocompromised patients
Support for timely clinical intervention

Sample Type & Reporting Output

Sample Types

Blood, Serum, Plasma

Reporting Output

Qualitative detection (Positive/Negative for BK virus)

Workflow Advantage

Single-tube assay reduces handling steps and simplifies workflow

Pre-optimized reagents ensure consistent amplification performance

Internal control integrated within the assay enables reliable validation

Closed-tube detection minimizes contamination risk

Compatible with standard real-time PCR instruments for routine laboratory use

Analytical Performance

Analytical Sensitivity:

100%

Analytical Specificity:

100%

Trueness (Bias):

98%

Accuracy:

99%

Limit of Detection (LOD):

100 copies/ml

Measuring Range:

10 – 10⁹ copies/reaction

Clinical Sensitivity:

98%

Clinical Specificity:

99%

Storage and Stability

Shelf life: 12 months from date of manufacture
Storage: -20°C (shipped at -30°C to -10°C)
Avoid repeated freeze-thaw cycles (>6 times)
Protect from direct sunlight

Applications & Use Scenarios

The kit is intended for use in clinical laboratories for:

Detection of BK virus infection
Monitoring viral reactivation
Routine molecular diagnostic workflows
Support for clinical decision-making

Ordering Information

Commercial Name	Old Cat No.	New Cat No.	Pack Size
BK-Q Real-Time PCR Kit for BK Virus	G2M705421		50 Tests

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Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.