C. Albicans-Q Real-Time PCR Kit

Qualitative Detection of Candida albicans DNA by Real-Time PCR

The C. Albicans-Q Real-Time PCR Kit enables qualitative detection of Candida albicans DNA in human clinical samples using real-time PCR. It provides a sensitive and reliable molecular method for detecting fungal infections in clinical laboratory settings.

At a Glance

Product Overview

The C. Albicans-Q Real-Time PCR Kit is a probe-based molecular assay designed for the specific detection of Candida albicans DNA.

The assay utilizes target-specific primers and fluorescent probes for amplification and detection of the pathogen. An internal control (RNaseP) is incorporated within the assay to monitor nucleic acid extraction efficiency and amplification performance, ensuring accurate and reliable results. A positive control is included to validate assay setup and reagent integrity.

C. Albicans-Q

How the Assay Works

  • Sample Collection & Nucleic Acid Extraction: DNA is extracted from clinical samples such as blood, genital swabs, or fluids using validated extraction methods.
  • Real-Time RT-PCR Amplification: Extracted DNA is amplified using Candida albicans-specific primers and probes.
  • Fluorescent Detection: Amplification signals are detected in real-time for both target and internal control, enabling accurate identification.
  • Result Interpretation: Ct value ≤ 35 is considered positive.

Key Features & Benefits

  • Specific detection of Candida albicans DNA
  • Probe-based assay for high specificity
  • Integrated internal control for assay validation
  • Positive control included for performance verification
  • Reliable qualitative detection
  • Suitable for routine clinical laboratory workflows

Why Candida albicans Testing Matters

Candida albicans is a common opportunistic fungal pathogen present as part of normal human microbiota but capable of causing infections under certain conditions.

Clinical relevance includes:
  • Leading cause of fungal infections globally (~70%)
  • Causes superficial infections such as oral thrush and vaginal candidiasis
  • Can lead to invasive systemic infections affecting multiple organs
  • High mortality (~40%) in immunocompromised patients with invasive infections
  • Common in hospital-acquired infections
Real-Time PCR enables:
  • Rapid and accurate detection
  • High sensitivity and specificity
  • Early diagnosis for improved treatment outcomes

Sample Type & Reporting Output

Sample Types
  • Blood, genital swabs, clinical fluids
Reporting Output
  • Qualitative detection (Positive/Negative)

Workflow Advantage

Single-tube assay reduces handling steps
Pre-formulated reagents simplify assay setup
Internal control integrated within assay design
Closed-tube system minimizes contamination risk
Compatible with standard real-time PCR instruments

Analytical Performance

Ct cut-off:
≤ 35
Positive Control Ct:
18 ± 3
No amplification in NTC confirms absence of contamination
Internal Control validates extraction and amplification efficiency

Storage and Stability

  • Shelf life: 12 months from date of manufacture
  • Storage: -20°C (shipped at -30°C to -10°C)
  • Perform assay at: 15°C to 30°C
  • Protect from direct sunlight

Applications & Use Scenarios

The kit is intended for use in clinical laboratories for:

  • Detection of Candida albicans infections
  • Fungal pathogen screening
  • Routine molecular diagnostic workflows
  • Supporting clinical diagnosis and patient management

Ordering Information

Commercial Name Old Cat No. New Cat No. Pack Size
C. Albicans-Q Real-Time PCR Kit G2M708021 50 Tests

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