Thrombosis-Q Comprehensive RT-PCR kit

Qualitative Detection of Thrombosis-Associated Genetic Mutations by Real-Time PCR

The Thrombosis-Q Comprehensive Real-Time PCR Kit is an in vitro diagnostic molecular assay designed for the qualitative detection of key genetic mutations associated with thrombosis using real-time PCR technology. The assay enables identification of clinically relevant mutations in human blood samples, supporting genetic risk assessment for thrombotic disorders.

At a Glance

Target: Thrombosis-associated genetic mutations (FII, FV Leiden, MTHFR, PAI-1)
Technology: Real-Time PCR (allele-specific probe-based detection)
Sample Type: Whole blood
Detection Type: Qualitative (Genotyping)
Application: Thrombophilia risk assessment

Product Overview

The Thrombosis-Q Comprehensive Real-Time PCR Kit is designed to detect multiple genetic mutations associated with thrombosis in a single workflow.

The kit utilizes multiple ready-to-use reaction mixes containing allele-specific primers and dual-labelled probes to differentiate between wild-type and mutant alleles. Detection is performed across multiple fluorescence channels, enabling accurate discrimination of genotypes. An internal control is included to ensure amplification performance and overall assay reliability.

How the Assay Works

The assay follows a structured molecular workflow:

Sample Collection & DNA Extraction: DNA is extracted from EDTA-treated whole blood samples using validated extraction methods.
Reaction Setup: Extracted DNA is added to ready-to-use reaction mixes containing allele-specific primers and probes.
Real-Time PCR Amplification: Target regions are amplified using real-time PCR.
Fluorescent Detection: Amplification signals are detected across multiple fluorescence channels.
Result Interpretation: Genotypes are determined based on amplification patterns across reaction mixes.

Key Features & Benefits

Detection of multiple thrombosis-associated mutations
Allele-specific real-time PCR for genotyping
Differentiation of wild-type, heterozygous, and mutant genotypes
Multi-channel fluorescence detection
Internal control for assay validation
Ready-to-use reaction mixes for simplified workflow

Why Thrombosis Genetic Testing Matters

Thrombophilia is a multifactorial condition influenced by genetic and environmental factors. Mutations such as Factor V Leiden and Prothrombin G20210A are well-established contributors to increased thrombotic risk.

Variants in the MTHFR gene may affect homocysteine metabolism, while PAI-1 polymorphisms influence fibrinolysis and clot stability. Identifying these mutations supports improved risk assessment and informed clinical decision-making.

Targets Coverage

Mutation Coverage

The assay detects the following clinically relevant mutations:

Factor II (FII): G20210A
Factor V Leiden (FV): G1691A
MTHFR: C677T
MTHFR: A1298C
PAI-1: 4G/5G polymorphism

Genotype Interpretation

Genotype determination is based on amplification patterns observed during real-time PCR:

Wild-Type: Amplification observed only in wild-type reaction
Heterozygous: Amplification observed in both wild-type and mutant reactions
Homozygous Mutant: Amplification observed only in mutant reaction

Comprehensive Panel Advantage

The assay enables simultaneous detection of multiple thrombosis-associated mutations in a single workflow, supporting efficient evaluation of combined genetic risk factors. This reduces the need for multiple individual tests and improves overall laboratory efficiency.

Sample Type & Reporting Output

Sample Types

Whole blood (EDTA)

Reporting Output

Qualitative genotyping (wild-type / heterozygous / homozygous mutant)

Analytical Performance

The assay demonstrates the following performance characteristics:

Consistent Ct Range:

21 ≤ Ct ≤ 26 (±2–3 cycles depending on extraction method)

Clear Genotype Discrimination:

Wild-type, heterozygous, and mutant profiles

Internal Control Ct Value:

≤ 26

Validated Controls:

Positive and no-template controls ensure assay reliability

Designed for Laboratory Efficiency

The assay is optimized for efficient molecular workflows:

Pre-formulated ready mixes reduce preparation steps
Structured workflow for multi-target detection
Clear interpretation based on amplification patterns
Closed-tube system minimizes contamination risk
Compatible with standard real-time PCR platforms

Storage and Stability

Shelf life: 12 months from date of manufacture
Storage temperature: -30°C to -10°C
Components stable at -20°C until expiry
Avoid repeated freeze-thaw cycles (>6 times)
Protect from direct sunlight

Applications & Use Scenarios

The kit is intended for use in clinical and research laboratories for:

Genetic screening of thrombophilia-associated mutations
Risk assessment of inherited thrombotic disorders
Evaluation of patients with thrombotic conditions
Identification of genetic predisposition to clotting disorders
Research in thrombosis and haemostasis

Ordering Information

Commercial Name	Old Cat No.	New Cat No.	Pack Size
Thrombosis-Q Comprehensive Real-Time PCR Kit	G2M803421		50 Tests

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