SCA-Q Real-Time PCR Kit

Qualitative Detection of Sickle Cell Anaemia (HbS Mutation) by Real-Time PCR

The SCA-Q Real-Time PCR Kit is an in vitro diagnostic molecular assay designed for the qualitative detection of the Sickle cell mutation in the human beta globin gene, using real-time PCR technology. The assay enables accurate genotyping from human genomic DNA, supporting screening, diagnosis, and genetic counselling.

At a Glance

Product Overview

The SCA-Q Real-Time PCR Kit is designed to detect the human beta globin GAG-GTG [Glu-Val] mutation, using allele-specific primers and fluorescent probes. The assay differentiates between wild-type and mutant alleles, enabling accurate identification of normal, carrier, and affected individuals.

The kit utilizes ready-to-use reaction mixes containing PCR buffer, Taq polymerase, specific primers, and probes. Fluorescence detection is performed using:

  • FAM channel: Sickle cell anaemia target (HbS mutation)
  • Texas Red channel: Wild-type gene (serves as internal control)

Amplification of the wild-type gene confirms the absence of PCR inhibition and ensures efficient DNA purification.

SCA-Q

How the Assay Works

The assay follows a structured molecular workflow:

  • Sample Collection & DNA Extraction: Genomic DNA is extracted from EDTA-treated whole blood samples using validated extraction methods.
  • Reaction Setup: Extracted DNA is added to reaction mixes containing allele-specific primers and probes.
  • Real-Time PCR Amplification: The HBB gene region is amplified using real-time PCR.
  • Fluorescent Detection: FAM detects HbS mutation
    Texas Red detects wild-type gene (internal control)
  • Result Interpretation: Genotype is determined based on amplification patterns.

Key Features & Benefits

  • Detection of HbS mutation associated with sickle cell anaemia
  • Allele-specific real-time PCR for genotyping
  • Differentiation of normal, carrier, and affected individuals
  • Probe-based detection for high specificity
  • Internal control for assay validation
  • Ready-to-use format for simplified workflow

Why Sickle Cell Genetic Testing Matters

Sickle Cell Anaemia is an inherited blood disorder caused by a mutation in the HBB gene, resulting in abnormal haemoglobin (HbS). This leads to distorted red blood cells, causing anaemia, pain episodes, and potential organ damage.

Early detection of the HbS mutation supports:
  • carrier screening
  • early diagnosis
  • genetic counselling
  • informed reproductive decisions

Mutation Coverage

Mutation Coverage

The assay detects the following mutation:

  • • human beta globin GAG-GTG [Glu-Val] mutation (HbS mutation)
Genotype Interpretation

Genotype determination is based on amplification patterns:

  • Wild-Type (Normal): Amplification in Texas Red channel only
  • Heterozygous (Carrier): Amplification in both FAM and Texas Red channels
  • Homozygous Mutant (Affected): Amplification in FAM channel only

Comprehensive Panel Advantage

The assay enables rapid and accurate detection of the HbS mutation in a streamlined workflow, supporting efficient genetic screening and reducing the need for conventional multi-step diagnostic methods.

Sample Type & Reporting Output

Sample Types
  • Whole blood (EDTA)
Reporting Output
  • Qualitative genotyping (wild-type / heterozygous / homozygous mutant)

Analytical Performance

The assay demonstrates the following performance characteristics:

Accurate Genotype determination based on amplification signals in FAM and Texas Red channels
Internal control (wild-type gene) confirms successful amplification and DNA quality
Clear differentiation between wild-type, heterozygous, and mutant profiles
Internal control validation ensures assay reliability

Designed for Laboratory Efficiency

The assay is optimized for streamlined workflows:

  • Ready-to-use reagents reduce preparation steps
  • Rapid turnaround time
  • Closed-tube detection minimizes contamination risk
  • Compatible with standard real-time PCR platforms

Storage and Stability

  • Shelf life: 12 months from date of manufacture
  • Storage temperature: -30°C to -10°C
  • Components stable at -20°C until expiry
  • Avoid repeated freeze-thaw cycles
  • Protect from direct sunlight

Applications & Use Scenarios

The kit is intended for use in clinical and research laboratories for:

  • Screening of sickle cell anaemia
  • Carrier detection
  • Genetic diagnosis of HbS mutation
  • Population screening programs
  • Research in hemoglobinopathies

Ordering Information

Commercial Name Old Cat No. New Cat No. Pack Size
SCA-Q Real Time PCR Kit for Sickle Cell Anemia G2M803221 50 Tests

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