HIV-Q Real-Time PCR Kit

Qualitative/Quantitative Detection of Human Immunodeficiency Virus Type- 1 (HIV-1) specific RNA by Real-Time RT-PCR:

The HIV-Q Real-Time PCR Kit is a molecular assay designed for accurate quantification of HIV-1 specific RNA in human plasma samples using real-time RT-PCR technology. It supports reliable viral load measurement for clinical monitoring, enabling informed treatment decisions, therapy evaluation, and long-term disease management.

At a Glance

Product Overview

The HIV-Q Real-Time PCR Kit targets a conserved region of the HIV genome using specific primers and fluorescent probes, enabling accurate detection and quantification. Fluorescence detection is performed in the HEX channel for HIV targets, while an internal control is detected in the Cy5 channel to validate extraction efficiency and amplification performance.

The kit includes four calibrated quantification standards (QS1–QS4), allowing generation of a standard curve for precise viral load determination. This ensures reliable and reproducible quantification across different runs.

HIV-Q

How the Assay Works

The assay follows a standardized molecular workflow:

  • Sample Collection & RNA Extraction: Viral RNA is extracted from human plasma samples using validated extraction methods.
  • Real-Time PCR Amplification: Extracted RNA is converted into complementary DNA (cDNA) which is amplified using HIV-specific primers in a thermal cycler.
  • Fluorescent Detection:Amplification signals are detected in real time:
    HEX channel: HIV target
    Cy5 channel: Internal control
  • Quantification: Viral load is calculated using a standard curve generated from QS1–QS4.

Why HIV Viral Load Testing Matters

HIV is a retrovirus that integrates into host cells and replicates using reverse transcription, leading to progressive immune system damage if untreated. Viral load measurement is a critical parameter in HIV management, as it reflects the level of viral replication in the body.

Quantitative HIV testing is essential for monitoring response to antiretroviral therapy (ART), detecting early treatment failure or viral rebound, assessing disease progression and supporting timely clinical decisions. Sensitive molecular detection methods such as RT-qPCR enable accurate measurement of low-level viremia, which is crucial for effective long-term disease management.

Sample Type & Reporting Output

Sample Types
  • Human plasma
Reporting Output
  • Quantitative HIV viral load (copies/ml or IU/ml)

Targets Detected

HIV-1 RNA
  • Detection of conserved genomic region using probe-based chemistry (HEX channel)
Internal Control
  • Monitors nucleic acid extraction and amplification efficiency (Cy5 channel)

Key Features & Benefits

  • Quantitative detection of HIV-1 RNA
  • One-step RT-qPCR workflow
  • High sensitivity for low viral load detection
  • Probe-based detection for high specificity
  • Internal control for assay validation
  • Quantification standards for accurate viral load estimation
  • Reproducible performance across runs

Analytical Performance

The assay demonstrates strong analytical performance characteristics:

Analytical Sensitivity:
100%
Analytical Specificity:
100%
Limit of Detection (LoD):
40 copies/ml (~25 IU/ml)
Accuracy:
99%
Measuring Range:
10 – 10⁹ copies/reaction
No Cross-Reactivity:
Observed with HCV, HBV, HSV

These parameters support reliable and reproducible viral load quantification.

Designed for Laboratory Efficiency

The assay is optimized for streamlined laboratory workflows:

  • One-step RT-qPCR reduces handling steps
  • Pre-formulated reagents simplify assay setup
  • Multiplex detection (target + internal control) in a single reaction
  • Closed-tube detection minimizes contamination risk
  • Compatible with standard real-time PCR workflows

Storage and Stability

  • Shelf life: 12 months from date of manufacture
  • Storage temperature: -30°C to -10°C
  • Components stable at -20°C until expiry
  • Avoid repeated freeze-thaw cycles (>6 times)
  • Protect from direct sunlight

Applications & Use Scenarios

The kit is intended for use in research laboratories for:

  • HIV viral load monitoring
  • Evaluation of antiretroviral therapy (ART) efficacy
  • Longitudinal monitoring of HIV infection
  • High-risk population screening
  • Molecular research involving HIV quantification

Ordering Information

Commercial Name Old Cat No. New Cat No. Pack Size
HIV-Q Real-Time PCR Kit G2M801221R 50 Tests

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Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.

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