HCV-Q Real-Time PCR Kit

Qualitative/Quantitative Detection of Hepatitis C Virus (HCV) RNA by Real-Time RT-PCR

The HCV-Q Real-Time PCR Kit is a molecular assay designed for accurate detection and quantification of Hepatitis C Virus (HCV) RNA in human plasma samples using real-time RT-PCR technology. It enables reliable viral load measurement for monitoring infection, assessing disease progression, and evaluating response to antiviral therapy.

At a Glance

Product Overview

The HCV-Q Real-Time PCR Kit targets a conserved region of the HCV genome using specific primers and fluorescent probes, enabling accurate detection and quantification. Fluorescence detection is performed in the FAM channel for HCV targets, while an internal control is detected in the Cy5 channel to validate nucleic acid extraction and amplification efficiency.

The kit includes four calibrated quantification standards (QS1–QS4), enabling generation of a standard curve for precise viral load determination. This ensures consistent and reproducible quantification across runs.

HCV-Q

How the Assay Works

The assay follows a standardized molecular workflow:

  • Sample Collection & DNA Extraction: Viral RNA is extracted from human plasma samples using validated extraction methods.
  • Real-Time PCR Amplification: Extracted RNA is reverse transcribed into complementary DNA (cDNA) and amplified using HCV-specific primers.
  • Fluorescent Detection: Amplification signals are detected in real time:
    FAM channel: HCV target
    Cy5 channel: Internal control
  • Quantification: Viral load is calculated using a standard curve generated from QS1–QS4.

Why HCV Viral Load Testing Matters

Hepatitis C is a viral infection that primarily affects the liver and can lead to chronic liver disease, cirrhosis, and hepatocellular carcinoma if untreated. It is mainly transmitted through exposure to infected blood.

Viral load measurement plays a critical role in HCV management by indicating the level of viral replication. Quantitative testing supports monitoring of antiviral therapy, assessment of treatment response, and evaluation of disease progression.

Sensitive molecular methods such as RT-qPCR enable accurate detection of low-level viremia, supporting effective disease management.

Sample Type & Reporting Output

Sample Types
  • Human plasma
Reporting Output
  • Quantitative HCV viral load (copies/ml or IU/ml)

Targets Detected

HCV RNA
  • Detection of conserved genomic region using probe-based chemistry (FAM channel)
Internal Control
  • Monitors nucleic acid extraction and amplification efficiency (Cy5 channel)

Key Features & Benefits

  • Quantitative detection of HCV RNA
  • One-step RT-qPCR workflow
  • High sensitivity for low viral load detection
  • Probe-based detection for high specificity
  • Internal control for assay validation
  • Quantification standards for accurate viral load estimation
  • Reproducible performance across runs

Analytical Performance

The assay demonstrates the following performance characteristics:

Analytical Sensitivity:
100%
Analytical Specificity:
100%
Trueness (Bias):
99%
Accuracy (Resulting from Trueness and Precision):
99%
Limit of Detection (LoD):
40 copies/ml (~25 IU/ml)
Measuring Range:
10 – 10⁹ copies/reaction
Slope of Quantification Standards:
-3.24
Interfering Substances:
Well controlled (Human genomic DNA, Anti-HBV drugs)
Cross-reactivity:
No cross-reactivity observed with HIV, HBV, HSV
Research Sensitivity:
99%
Research Specificity:
99%

These parameters support reliable and reproducible viral load quantification.

Designed for Laboratory Efficiency

The assay is optimized for streamlined laboratory workflows:

  • One-step RT-qPCR reduces handling steps
  • Pre-formulated reagents simplify assay setup
  • Multiplex detection (target + internal control) in a single reaction
  • Closed-tube detection minimizes contamination risk
  • Compatible with standard real-time PCR workflows

Storage and Stability

  • Shelf life: 12 months from date of manufacture
  • Storage temperature: -30°C to -10°C
  • Components stable at -20°C until expiry
  • Avoid repeated freeze-thaw cycles (>6 times)
  • Protect from direct sunlight

Applications & Use Scenarios

The kit is intended for use in research laboratories for:

  • HCV viral load monitoring
  • Evaluation of antiviral therapy efficacy
  • Longitudinal monitoring of HCV infection
  • High-throughput molecular testing workflows
  • Molecular research involving HCV quantification

Ordering Information

Commercial Name Old Cat No. New Cat No. Pack Size
HCV-Q Real-Time PCR Kit G2M801221R 50 Tests

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Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.

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