HBV-Q Real Time PCR Kit

Qualitative/Quantitative Detection of Hepatitis B Virus (HBV) DNA by Real-Time PCR

The HBV-Q Real-Time PCR Kit is a molecular assay designed for accurate detection and quantification of Hepatitis B Virus (HBV) DNA in human plasma or serum samples using real-time PCR technology. It enables reliable viral load measurement for assessing infection status, monitoring disease progression, and evaluating response to antiviral therapy.

At a Glance

Product Overview

The HBV-Q Real-Time PCR Kit targets a conserved region of the HBV genome using specific primers and fluorescent probes, enabling accurate detection and quantification. Fluorescence detection is performed in the Texas Red channel for HBV targets, while an internal control is detected in the Cy5 channel to validate nucleic acid extraction and amplification efficiency.

The kit includes four calibrated quantification standards (QS1–QS4), allowing generation of a standard curve for precise viral load determination, ensuring reliable and reproducible quantification across different runs.

HBV-Q

How the Assay Works

The assay follows a standardized molecular workflow:

  • Sample Collection & DNA Extraction: Viral DNA is extracted from human plasma or serum samples using validated extraction methods.
  • Real-Time PCR Amplification: Extracted DNA is amplified using HBV-specific primers in a thermal cycler.
  • Fluorescent Detection: Amplification signals are detected in real time:
    Texas Red channel: HBV target
    Cy5 channel: Internal control
  • Quantification: Viral load is calculated using a standard curve generated from QS1–QS4.

Why HBV Viral Load Testing Matters

Hepatitis B is a viral infection that affects the liver and can lead to both acute and chronic disease. It is transmitted through exposure to infected blood and body fluids, including perinatal transmission, needle injuries, and other routes.

Viral load measurement is a critical parameter in HBV management, as it reflects the level of viral replication and helps guide clinical decisions. Quantitative HBV testing supports monitoring of antiviral therapy, assessment of disease progression, and evaluation of treatment response.

Sample Type & Reporting Output

Sample Types
  • Human plasma / serum
Reporting Output
  • Quantitative HBV viral load (copies/ml or IU/ml)

Targets Detected

HBV DNA
  • Detection of conserved genomic region using probe-based chemistry (Texas Red channel)
Internal Control
  • Monitors nucleic acid extraction and amplification efficiency (Cy5 channel)

Key Features & Benefits

  • Quantitative detection of HBV DNA
  • Probe-based real-time PCR assay
  • High sensitivity for low viral load detection
  • Internal control for assay validation
  • Quantification standards for accurate viral load estimation
  • Reproducible performance across runs

Analytical Performance

The assay demonstrates the following performance characteristics:

Analytical Sensitivity:
100%
Analytical Specificity:
100%
Trueness (Bias):
99%
Accuracy (Resulting from Trueness and Precision):
99%
Limit of Detection (LoD):
70 copies/ml (~12.0 IU/ml)
Measuring Range:
10 – 10⁹ copies/reaction
Slope of Quantification Standards:
-3.47
Interfering Substances:
Well controlled (Human genomic DNA, Anti-HBV drugs)
Cross-reactivity:
No cross-reactivity observed with HIV, HCV, HSV
Research Sensitivity:
99%
Research Specificity:
99%

These parameters support reliable and reproducible viral load quantification.

Designed for Laboratory Efficiency

The assay is optimized for streamlined laboratory workflows:

  • Pre-formulated reagents simplify assay setup
  • Standardized workflow for consistent performance
  • Multiplex detection (target + internal control) in a single reaction
  • Closed-tube detection minimizes contamination risk
  • Compatible with standard real-time PCR workflows

Storage and Stability

  • Shelf life: 12 months from date of manufacture
  • Storage temperature: -30°C to -10°C
  • Components stable at -20°C until expiry
  • Avoid repeated freeze-thaw cycles (>6 times)
  • Protect from direct sunlight

Applications & Use Scenarios

The kit is intended for use in research laboratories for:

  • HBV viral load monitoring
  • Evaluation of antiviral therapy efficacy
  • Longitudinal monitoring of HBV infection
  • High-throughput molecular testing workflows
  • Molecular research involving HBV quantification

Ordering Information

Commercial Name Old Cat No. New Cat No. Pack Size
HBV-Q Real-Time PCR Kit G2M801221R 50 Tests

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Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.

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