3HDtect-Q Real Time PCR Kit

Qualitative Detection of Human Immunodeficiency Virus (HIV), Hepatitis C Virus (HCV), and Hepatitis B Virus (HBV) by Real-Time RT-PCR

The 3HDtect-Q Real-Time PCR Kit is a multiplex molecular assay designed for the simultaneous qualitative detection of HIV, HCV, and HBV nucleic acids in human plasma samples using real-time RT-PCR technology. It enables efficient screening of major blood-borne viral pathogens in a single reaction, supporting streamlined testing workflows and improved laboratory efficiency.

At a Glance

Target: HIV, HCV, HBV
Technology: Multiplex Real-Time RT-PCR (probe-based chemistry)
Sample Type: Human plasma
Detection Type: Qualitative
Application: Multiplex viral screening

Product Overview

The 3HDtect-Q Real-Time PCR Kit enables simultaneous detection of three clinically significant viruses; HIV, HCV, and HBV, within a single reaction using target-specific primers and fluorescent probes.

RNA viruses (HIV and HCV) are reverse transcribed into complementary DNA (cDNA), while HBV DNA is directly amplified. Detection is achieved using probe-based fluorescence across distinct channels, allowing accurate identification of each target without cross-interference. An internal control is included to monitor nucleic acid extraction and amplification efficiency, ensuring the validity and reliability of test results.

How the Assay Works

The assay follows a standardized multiplex molecular workflow:

Sample Collection & Nucleic Acid Extraction: Viral RNA/DNA is extracted from human plasma samples using validated extraction methods.
Real-Time PCR Amplification: Target-specific primers amplify HIV, HCV, and HBV nucleic acids simultaneously.
Fluorescent Detection: Amplification signals are detected in real time using probe-based chemistry for each target.
Result Interpretation: Presence or absence of amplification signals determines qualitative detection of each virus.

Why Multiplex Viral Testing Matters

HIV, HBV, and HCV are major bloodborne pathogens associated with chronic infections, immunodeficiency, and liver disease. Co-infections are common, particularly in transfusion, transplant and high-risk populations, making simultaneous detection essential for effective screening and management.

Multiplex RT-PCR enables detection of multiple pathogens in a single reaction, reducing turnaround time, minimizing reagent usage, and improving laboratory efficiency while maintaining high sensitivity and specificity.

Sample Type & Reporting Output

Sample Types

Human plasma

Reporting Output

Qualitative detection of HIV, HCV, and HBV

Targets Detected

Viral Target

HIV-1 RNA: detected in HEX channel
HCV RNA: detected in FAM channel
HBV DNA: detected in Texas Red channel

Internal Control

Monitors nucleic acid extraction and amplification efficiency

Key Features & Benefits

Simultaneous detection of HIV, HCV, and HBV
Multiplex real-time RT-PCR assay
High sensitivity and specificity
Probe-based detection for accurate target identification
Internal control for assay validation
Reduced turnaround time and improved workflow efficiency
Suitable for high-throughput molecular testing

Analytical Performance

The assay demonstrates reliable multiplex detection performance:

Analytical Sensitivity:

100%

Analytical Specificity:

100%

Trueness (Bias):

99%

Accuracy (Resulting from Trueness and Precision):

99%

Limit of Detection (LoD):

40 copies/ml

Measuring Range:

10 – 10⁹ copies/reaction

Slope of Quantification Standards:

~ -3.2 to -3.3

Interfering Substances:

Well controlled (Human genomic RNA, Anti-HIV drugs)

Cross-reactivity:

No cross-reactivity observed with HCV, HBV, HSV

Research Sensitivity:

97%

Research Specificity:

99%

Designed for Laboratory Efficiency

The assay is optimized for streamlined workflows:

Single-reaction detection of three pathogens
Reduced handling steps and reagent consumption
Multiplex workflow improves throughput
Closed-tube detection minimizes contamination risk
Compatible with standard real-time PCR workflows

Storage and Stability

Store kit components as per recommended conditions
Avoid repeated freeze-thaw cycles
Maintain cold chain during storage and handling

Applications & Use Scenarios

The kit is intended for use in research laboratories for:

Multiplex screening of HIV, HBV, and HCV
Bloodborne pathogen detection workflows
High-throughput infectious disease screening
Screening of high-risk populations
Routine molecular testing workflows

Ordering Information

Commercial Name	Old Cat No.	New Cat No.	Pack Size
3HDtect-Q Real Time PCR Kit	G2M801221R		50 Tests

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Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.