Overview

The SpiNXT FFPE Nucleic Acid Extraction Kit utilizes silica membrane spin column technology to efficiently purify nucleic acids from FFPE tissue sections. Tissue sections are first deparaffinized and lysed using optimized buffers, which enable release of DNA and RNA while inactivating nucleases and denaturing proteins. Under high-salt conditions, nucleic acids selectively bind to the silica membrane, allowing separation from proteins, paraffin residues, and other contaminants. Sequential wash steps remove impurities, and DNA and RNA are eluted in a low-salt buffer or nuclease-free water. The workflow eliminates the need for hazardous organic solvents such as phenol or chloroform and avoids alcohol precipitation. The extracted DNA and RNA are fragmented yet suitable for amplification-based assays, sequencing, and molecular analyses, making them compatible with a wide range of downstream applications.