SpiNXT DBS DNA Extraction Kit

The SpiNXT DBS DNA Extraction Kit (Dried Blood Spot) is designed for the isolation of genomic DNA from dried blood spot samples collected on filter paper or approved specimen collection cards. The kit supports reliable DNA recovery from limited and dried blood samples, enabling their use in routine molecular biology and genetic analysis workflows where DBS sampling is preferred.

Overview

The SpiNXT DBS DNA Extraction Kit is based on a silica membrane based purification method combined with optimized enzymatic lysis for the extraction of genomic DNA from dried blood spot samples. Blood cells immobilized on filter paper are lysed to release DNA, which selectively binds to the silica membrane under appropriate buffer and salt conditions. Following binding, proteins, inhibitors, and other contaminants are removed through sequential wash steps. The DNA is subsequently eluted in a low-salt buffer, resulting in purified genomic DNA suitable for downstream molecular biology applications. The workflow is streamlined, requires minimal handling and centrifugation, and does not involve the use of hazardous organic solvents such as phenol or chloroform or alcohol precipitation.

Product Features

Recommended Input Amount: 3 x 3 mm diameter punches
Binding Capacity: 30-50μg genomic DNA
Elution Volume: ≥50μl
Purity: A260/280 - 1.8±0.1, A260/230 - 2.0±0.1
Compatible Downstream Applications : Microarray analysis, STR analysis, restriction enzyme digestion & Southern blotting
Expected Yield: >600ng or 0.6 µg

Applications

Genetic Screening & Molecular Testing

DNA extracted from DBS samples can be used for genetic screening and mutation detection in research and molecular testing workflows.
Suitable for applications where sample volume is limited or remote sample collection is required.

Microarray Analysis

Purified genomic DNA is suitable for hybridization-based microarray assays, enabling genome-wide analysis such as copy number variation and gene profiling.
Compatible with workflows requiring high-purity DNA and consistent performance.

STR Analysis

Extracted DNA can be used for Short Tandem Repeat (STR) analysis, commonly applied in genetic identification and population-based studies.
Supports reliable amplification of repeat regions from DBS-derived DNA.

Restriction Enzyme Digestion

The purified DNA is compatible with restriction endonuclease digestion, enabling downstream applications such as cloning, mapping, and molecular characterization.
Absence of inhibitors ensures efficient enzymatic activity.

Southern Blotting

Suitable for Southern blot analysis, where high-quality genomic DNA is required for restriction digestion, gel electrophoresis, and membrane hybridization.
Enables detection of specific DNA sequences within complex genomes.

Panel Specifications

Format:

Mini spin column

Technology:

Silica membrane

Sample Type:

Filter With Dried Blood

Yield:

Up to 10 µg

Target:

Genomic DNA

A260/280:

1.6–1.9

Available Pack Sizes:

50 T / 250 T

Throughput Compatibility:

Manual

Commercial Name	Cat No.	Pack Size
SpiNXT DBS DNA Extraction Kit	G2MBR4-0591	50 T
SpiNXT DBS DNA Extraction Kit	G2MBR4-0592	250 T

Commercial Name

Cat No.

Pack Size

SpiNXT DBS DNA Extraction Kit

G2MBR4-0591

50 T

SpiNXT DBS DNA Extraction Kit

G2MBR4-0592

250 T

Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.

All products listed in the catalogue are the products of Genes2Me Private Limited. Apart from that, all other product names, trademarks and logos wherever used in the catalogue are the property of their respective owners.

SpiNXT DBS DNA Extraction Kit

Overview

Product Features