MagNXT Bacterial DNA Extraction

MagNXT Bacterial DNA Extraction Kit

The MagNXT Bacterial DNA Extraction Kit is a high-quality, magnetic bead-based solution developed for the rapid and efficient isolation of genomic DNA from bacterial cells. Optimized for both Gram-positive and Gram-negative bacteria, the kit delivers consistent yield and purity, making it suitable for routine laboratory use as well as advanced molecular workflows.

Kit components: Magnetic Bead Particles | Buffer DLB | Buffer DLB-20 | Buffer W1 | Buffer W2 | Proteinase K | Proteinase Dissolving Buffer | Buffer EB | RNaseA| Lysozyme

Blood DNA Extraction

Overview


The MagNXT Bacterial DNA Extraction Kit combines optimized cell lysis chemistry with magnetic bead-based DNA purification to ensure reliable and reproducible extraction of high-quality bacterial genomic DNA. Bacterial cells are efficiently lysed using enzymatic and chemical reagents, enabling complete release of genomic DNA. The released DNA selectively binds to magnetic beads under optimized conditions, while proteins, salts, and other cellular contaminants are effectively removed through successive wash steps. Purified DNA is finally eluted in a low-salt buffer or water, resulting in DNA that is immediately compatible with downstream molecular applications. The protocol eliminates the use of hazardous organic solvents such as phenol-chloroform or alcohol precipitation, offering a safe, fast, and user-friendly workflow. This streamlined approach ensures minimal hands-on time, high reproducibility, and reliable performance across a wide range of bacterial sample types.

Product Features


BActerial DNA Extraction Kits
  • Recommended Input Amount: ~1.2ml of bacterial culture
  • Binding Mechanism: Super paramagnetic beads
  • Elution Volume: 30 - 50 µl
  • Purity: A260/280 1.8±0.1, A260/230 -2.0±0.1
  • Expected Yield: ≥5μg (Depending upon the quality & quantity of the starting material used)

Applications


PCR and qPCR
  • Reliable amplification and quantification of bacterial genomic DNA.
Next-Generation Sequencing
  • High-quality DNA suitable for library preparation and sequencing analysis.
Cloning and Genetic Engineering
  • Supports gene cloning, construct development, and molecular biology workflows.
Genotyping and Strain Identification
  • Enables accurate bacterial strain typing and genetic characterization.
Microbial Research and Diagnostics
  • Ideal for academic research, applied microbiology, and clinical laboratory studies.

Panel Specifications


Format: Paramagnetic Beads
Technology: Magnetic Bead Base
Sample Type: Bacterial Culture
Yield: ≥5μg
Target: Bacterial DNA
A260/280: 1.6–1.9
Available Pack Sizes: 50 T, 250 T
Throughput Compatibility: Manual

Workflow / How It Works


Extraction workflow

Data Analysis and Reporting


Bacterial DNA Extraction
DNA Bioanalyzer

Ordering Information


Commercial Name Cat No. Pack Size
MagNXT Bacterial DNA Extraction Kit G2MBR4-0646 50 T
MagNXT Bacterial DNA Extraction Kit G2MBR4-0647 250 T

Product Enquiry Form


Resources to get started


Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.

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