YCM-Q Real Time PCR Kit

Qualitative Detection of Y Chromosome Microdeletions by Real-Time PCR

The YCM-Q Real-Time PCR Kit is an in vitro diagnostic molecular assay designed for the qualitative detection of microdeletions in the Y chromosome using real-time PCR technology. The assay enables identification of deletions in key regions associated with male infertility, supporting genetic evaluation and clinical decision-making.

At a Glance

Product Overview

The YCM-Q Real Time PCR Kit for Y Chromosome Microdeletion is an in vitro diagnostic test designed to detect deletions at 15 different regions of Y Chromosome. These regions are essential for spermatogenesis, and deletions within them are strongly associated with male infertility.

The assay utilizes target-specific primers and fluorescent probes for each region, enabling simultaneous detection in a multiplex real-time PCR format. Detection is performed across multiple fluorescence channels, while an internal control ensures amplification efficiency and assay validity.

YCM-Q

How the Assay Works

The assay follows a structured molecular workflow:

  • Sample Collection & DNA Extraction: Genomic DNA is extracted from whole blood samples using validated extraction methods.
  • Reaction Setup: Extracted DNA is added to reaction mixes containing region-specific primers and probes.
  • Real-Time PCR Amplification: Target regions within the Y chromosome are amplified using real-time PCR.
  • Fluorescent Detection: Amplification signals are detected across multiple fluorescence channels corresponding to specific regions.
  • Result Interpretation: Presence or absence of amplification determines deletion status in each targeted region.

Key Features & Benefits

  • Detection of key Y chromosome microdeletion regions
  • Multiplex real-time PCR assay
  • High specificity using probe-based detection
  • Internal control for assay validation
  • Efficient workflow for genetic screening
  • Reliable and reproducible results

Why Y Chromosome Microdeletion Testing Matters

Microdeletions in the Y chromosome are one of the most common genetic causes of male infertility, particularly in cases of azoospermia and severe oligozoospermia. The AZF regions (AZFa, AZFb, AZFc and AZFd) contain genes critical for sperm production.

Detection of these deletions supports:
  • identification of genetic causes of infertility
  • guidance for assisted reproductive techniques
  • genetic counselling and patient management
  • informed clinical decision-making

Mutation Coverage

Mutation Coverage

The assay detects microdeletions in the following 15 regions of Y Chromosome:

  • SY14 (Control), ZFY (Control), SY84 (AZFa), SY127 (AZFb),
  • SY255 (AZFc/DAZ), SY86 (AZFa), SY254 (AZFc/DAZ), SY134 (AZFb),
  • SY81 (AZFa), SY164 (AZFb), SY152 (AZFc/d), SY145 (AZFc/d),
  • SY153 (AZFc/d), SY277(AZFc-DAZ) and SY142 (AZFb)
Genotype Interpretation

Interpretation is based on amplification patterns observed during real-time PCR:

  • No Deletion (Normal): Amplification observed for all targeted regions
  • Microdeletion Present: Absence of amplification in one or more specific regions
  • Invalid Result: Absence of internal control amplification

Comprehensive Panel Advantage

The assay enables simultaneous analysis of multiple AZF regions in a single reaction, allowing comprehensive evaluation of Y chromosome integrity. This reduces the need for multiple individual assays and improves workflow efficiency in genetic testing laboratories.

Sample Type & Reporting Output

Sample Types
  • Whole blood
Reporting Output
  • Qualitative detection of Y chromosome microdeletions (region-wise)

Analytical Performance

The assay demonstrates the following performance characteristics:

High analytical sensitivity and specificity for targeted regions
Accurate detection of deletions across AZF regions
Consistent amplification performance across runs
Internal control validation ensures assay reliability
Clear differentiation between deletion and non-deletion profiles

Designed for Laboratory Efficiency

The assay is optimized for streamlined workflows:

  • Multiplex detection of multiple regions in a single reaction
  • Reduced handling steps and reagent use
  • Closed-tube detection minimizes contamination risk
  • Compatible with standard real-time PCR platforms

Storage and Stability

  • Shelf life: 12 months from date of manufacture
  • Storage temperature: -30°C to -10°C
  • Components stable at -20°C until expiry
  • Avoid repeated freeze-thaw cycles (>6 times)
  • Protect from direct sunlight

Applications & Use Scenarios

The kit is intended for use in clinical and research laboratories for:

  • Genetic screening of male infertility
  • Detection of Y chromosome microdeletions
  • Evaluation of azoospermia and oligozoospermia cases
  • Pre-ART genetic assessment
  • Research in reproductive genetics

Ordering Information

Commercial Name Old Cat No. New Cat No. Pack Size
YCM-Q Real Time PCR Kit for Y Chromosome Micro deletion G2M803021 50 Tests

Product Enquiry Form

Other Products

Rapi-Q Series
Rapi-Q

Rapid and Compact Point-of-care RT-PCR Testing Solution.

Rapi-X96
RAPiCycler-96

Real time PCR system to process 96 samples.
TRapi-X96
Rapi-X16

Compact Nucleic Acid Extraction System Ideal for Point-of-Care Testing

Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.

© 2025 Genes2me. All rights reserved.