Molecular Biology Solutions/Products/Kits | DNA RNA RT-PCR Enzyme Polymerase

Fantom High- Fidelity PCR DNA Polymerase & Master mix

Fantom Hi-Fidelity DNA Polymerase is a new generation superior fidelity enzyme with 53 times more accurate

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in compare to conventional Taq DNA Polymerase and 6 times more than that of Pfu DNA Polymerase. While specificity-promoting factors contribute high-fidelity of the enzyme; association of elongation-factor promotes quicker amplification (0.5sec/Kb) of larger plasmid or DNA fragments (40Kb) as well as c-DNA up to 20kb. Furthermore, the enzyme's capacity to amplify varied GC content fragments and strong tolerance to PCR inhibitors distinguishes it from other enzymes when it comes to amplifying products from distinct species. The enzyme-mediated amplification gives blunt-ended products suitable for Fast-cloning. The product is also available in master mix format with dye.

Fast Cloning Kits

Our product line for fast cloning, The ReplicaExpress series is a new generation of recombinant cloning

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kits that can clone 1-5 fragments to any place in any vector backbone in as little as 5-15 minutes. The operating principle is homologous recombination between the insert and the kit-provided vector, regardless of the presence of A at the 3'-end. The simple workflow enables the kit to efficiently clone 50 bp～10 kb fragments seamless, with a positive rate of >95%.

Sample collection kits and Devices

Saliva collection kits are perfect for any DNA or RNA analysis research project. These tools give researchers

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an all-in-one solution for collecting, stabilising, transporting, and storing high-quality DNA or RNA samples in a reliable, non-invasive manner.

Conventional PCR and RT-PCR reagents

Depending on the needs, a broad range of qPCR and qRT-PCR products are available in the form of kits, master

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mixes, and standalone items. The advance cDNA Synthesis Kit has considerably enhanced heat stability and is ideal for reverse transcription of RNA templates with complicated secondary structures. In order to perform quantitative PCR in a single-tube format, Genes2Me incorporates NeoScript One Step qRT-PCR kit, which combines the functions of Reverse Transcriptase and DNA-polymerase. As a result, the RNA template is employed immediately for quantification, which minimises the number of stages in the experiment and makes it less arduous while preserving sensitivity.

TA CLONING

T4-DNA ligase and Rapi-5 Universal Ligation mix are two potential products that aid in cloning by speeding up

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the ligation procedure. On double-stranded DNA, the remarkably pure enzyme catalyses the formation of an ester bond between neighbouring 5'-phosphate ends and 3'-hydroxyl ends. Furthermore, in the Ligation mix format, which includes an optimised connection enhancer and reaction buffer, the enzyme's effectiveness is considerably improved. A variety of chemically competent cells can be directly transformed using the ligation product.

Isothermal amplification Reagents

Isothermal amplification technologies detect a nucleic acid target sequence in a simplified, exponential way

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that is not limited by thermal cycle constraints. For separating duplex DNA, the approach commonly uses distinct DNA polymerases. We have Large Fragment of Bst DNA Polymerase in this range. The enzyme has strong strand displacement activity and can be utilised for isothermal amplification reactions, RCA (Rolling-circle amplification), and other applications.

Hot Shot DNA Polymerase & master mix

Genes2me introduces Hot Shot DNA polymerase to prevent non-specific amplification and primer-dimerization

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during sample processing. The enzyme's rigorous activity is attributed to chemical alteration. As a result, the use of Hot Shot DNA polymerase in the experiment workflow provides excellent sensitivity by amplifying low-copy genes. Since the enzyme inserts Adenosine (A) at the 3’-end, the amplified product can be cloned into the T Vector. Hot Shot DNA polymerase, like Fantom Hi-Fidelity DNA polymerase, comes with a distinct master mix.

Markers & Ladders

Genes2me offers updated versions of markers to analyse the weight of DNA and Proteins more precisely

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in electrophoresis. Ladders of different sizes are available in pre-stained formats in our product range.

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Fantom High- Fidelity PCR DNA Polymerase & Master mix

Fantom Hi-Fidelity DNA Polymerase is a new generation superior fidelity enzyme with 53 times more accurate

Fast Cloning Kits

Our product line for fast cloning, The ReplicaExpress series is a new generation of recombinant cloning

Sample collection kits and Devices

Saliva collection kits are perfect for any DNA or RNA analysis research project. These tools give researchers

an all-in-one solution for collecting, stabilising, transporting, and storing high-quality DNA or RNA samples in a reliable, non-invasive manner.

Conventional PCR and RT-PCR reagents

Depending on the needs, a broad range of qPCR and qRT-PCR products are available in the form of kits, master

TA CLONING

T4-DNA ligase and Rapi-5 Universal Ligation mix are two potential products that aid in cloning by speeding up

Isothermal amplification Reagents

Isothermal amplification technologies detect a nucleic acid target sequence in a simplified, exponential way

Hot Shot DNA Polymerase & master mix

Genes2me introduces Hot Shot DNA polymerase to prevent non-specific amplification and primer-dimerization

Markers & Ladders

Genes2me offers updated versions of markers to analyse the weight of DNA and Proteins more precisely

in electrophoresis. Ladders of different sizes are available in pre-stained formats in our product range.

Available Products