Isothermal amplification Reagents

Isothermal amplification technologies detect a nucleic acid target sequence in asimplified, exponential way that is not limited by thermal cycle constraints. The conventional approach for separating duplex DNA uses distinct DNA polymerases.

Genes2Me offers ‘Bst DNA Polymerase’ derived from Bacillus Stearothermophilus, which is a large fragment DNA polymerase with strong strand displacement activity. It has 5’>3’ polymerase activity but lacks 5’>3’ exonuclease activity. The enzyme can be used for isothermal amplifications like loop-mediated isothermal amplification (LAMP), rolling-circle amplification (RCA ) etc.

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