The ReplicaExpress is an innovative recombinant cloning kit which facilitates quick and directional cloning of targeted fragments to any site in any vector backbone. It is a one-step multi-fragment seamless cloning technique that can clone up to 5 fragments of size 20 bp – 10 kb in 5 – 30 minutes, with a positive rate of >95%.
The kit works on the principle of homologous recombination between the insert and the vector (provided in the kit), where restriction enzyme direction is unnecessary and the reaction is independent of ligase and phosphatase activity.
