This kit enables efficient purification of these circulating nucleic acids from human plasma & serum utilizing Magnetic bead particles that bind DNA under optimized binding conditions. Utilizing circulating Cell-free DNA (cf-DNA) can serve as biomarker and provides real time mutational information that can be used to diagnose/prognosis & monitor the therapy for several cancer types and autoimmune diseases.
Magnetic Bead Particles | Buffer CLB-3 | Binding Solution-3 | Buffer EB | Proteinase K | Protease Dissolve Buffer | Buffer CW2
Recommended Input Amount : ~500μl Plasma/Serum (Depends upon the type of sample used)
Binding Mechanism : Super paramagnetic beads
Elution Volume : ≥30μl
Purity : A260/280 -1.8±0.1, A260/280 -2.0±0.1
Expected Yield : ≥0.6μg Depending upon the type, quality & quantity of the starting material used
PCR
qPCR
Microarrays
Next Generation Sequencing
Format |
Paramagnetic Beads |
Technology |
Magnetic bead |
Sample Type |
Plasma & Serum. |
Yield |
>0.6 µg (depending upon the type, quality & quantity of the starting material used) |
Target |
Circulating Cell Free DNA |
A260/280 |
1.6 - 1.9 |
Available in pack sizes |
50 Rxn, 250 Rxn |
Throughput Compatibility |
Manual and automation both |
CAT # |
DESCRIPTION |
UNITS |
G2M212121-CF |
MagNXT Tissue & Body Fluid DNA Extraction Kit (Cell Free DNA) |
50 Preps |
G2M212021-CF |
MagNXT Tissue & Body Fluid DNA Extraction Kit (Cell Free DNA) |
250 Preps |