This kit enables efficient purification of these circulating nucleic acids from human plasma, serum, or urine utilizing Magnetic bead particles that bind DNA under optimized binding conditions. Utilizing circulating Cell-free DNA (cf-DNA) can serve as biomarker and provides real time mutational information that can be used to diagnose/prognosis & monitor the therapy for several cancer types and autoimmune diseases.
Magnetic Bead Particles | Buffer CLB-3 | Binding Solution-3 | Buffer EB | Proteinase K | Protease Dissolve Buffer | Buffer CW2
Recommended Input Amount : ~500μl Plasma/Serum/Urine (Depends upon the type of sample used)
Binding Mechanism : Super paramagnetic beads
Elution Volume : ≥30μl
Purity : A260/280 -1.8±0.1, A260/280 -2.0±0.1
Expected Yield : ≥0.6μg Depending upon the type, quality & quantity of the starting material used
PCR
qPCR
Microarrays
Next Generation Sequencing
Format |
Pre-filled Plates |
Technology |
Magnetic bead |
Sample Type |
Plasma, Serum & Urine. |
Yield |
>0.6 µg (depending upon the type, quality & quantity of the starting material used) |
Target |
Circulating Cell Free DNA |
A260/280 |
1.6 - 1.9 |
Available in pack sizes |
96 / 192 / 480 preps |
Throughput Compatibility |
Automation |
CAT # |
DESCRIPTION |
UNITS |
G2M211921-CF(PF)R96 |
MagNXT Tissue & Body Fluid DNA Extraction Kit (Pre-filled for Rapi-X96 for Cell Free DNA) |
480 Preps |
G2M211921-CF(PF)R16 |
MagNXT Tissue & Body Fluid DNA Extraction Kit (Pre-filled for Rapi-X16 for Cell Free DNA) |
192 Preps |
G2M211921-CF(PF)R8 |
MagNXT Tissue & Body Fluid DNA Extraction Kit (Pre-filled for Rapi-X16 of 8 isolation each for Cell Free DNA) |
96 Preps |
The SpiNXT Tissue and Body Fluid DNA Extraction kit is a comprehensive solution optimized for cell/tissue lyses and purification of intact genomic DNA (gDNA) from a broad range of biological samples such as mammalian cells and tissues, Product of conception, chorionic villi sample, Cell culture pellet, Amniotic fluid, bronchial washings, Urine, sputum, saliva, pleural fluids, vaginal swabs, bronchoalveolar lavage (BAL) & other body fluids.
gDNA Mini Columns | Collection Tubes | Buffer TLB | Buffer TW1 | Buffer TW2 | Proteinase K | Protease Dissolve Buffer | LBTC Buffer | Buffer AE
Recommended Input Amount : ~ 25mg tissue/200µl for body fluids
Binding Capacity : 30-40μg genomic DNA
Elution Volume : ≥30μl
Purity :-1.8±0.1 A260/230 -2.0±0.1
Compatible Downstream Applications : AFLP, RFLP, Southern Blotting, Endpoint PCR, qPCR, Sequencing, etc
Expected Yield : ≥5μg (depending upon the type, quantity of the starting material used
AFLP
RFLP
Southern Blotting
Endpoint PCR
qPCR
Sequencing
Format |
Mini spin column |
Technology |
Silica membrane |
Sample Type |
Mammalian cells and tissues, Product of conception, chorionic villi sample, Cell culture pellet, Amniotic fluid, bronchial washings, Urine, sputum, saliva, pleural fluids, vaginal swabs, bronchoalveolar lavage (BAL), & other body fluids. |
Yield |
≥5μg |
Target |
Genomic DNA |
A260/280 |
1.6 - 1.9 |
Available in pack sizes |
50 Rxn, 250 Rxn |
Throughput Compatibility |
Manual |
CAT # |
DESCRIPTION |
UNITS |
G2M131420 |
SpiNXT Tissue & Body Fluids DNA Extraction Kit |
50 Rxn |
G2M131320 |
SpiNXT Tissue & Body Fluids DNA Extraction Kit |
250 Rxn |
