Liquid Biopsy Panels

Liquid Biopsy Panels

Oncological investigation in a cancer patient plays a significant role in order to develop an effective treatment plan. Comprehensive information related to tumors is required when associated genes, mutations and variants are investigated. Solid tumor biopsy though effective, has its own challenges. Tumor sample collection is a painful and costly process making this investigation difficult. Liquid biopsy is a method where circulating tumor DNA (ctDNA) shed from the tumor is extracted and sequenced. This unveils the necessary information regarding cancer causing mutations in the related genes enhancing clinicians to decide the best course of treatment.

Panel Overview

G2M offers a panel for liquid biopsy covering major cancer types like Lung, Breast and Colorectal cancer. This panel gives a detailed insight on cancer related genes to uncover known as well as novel variants for further investigation

ctDNA Breast Cancer Panel

A hybridization based solution for targeted sequencing employing NGS. With a fast turnaround time this product provides detection and identification of 36 clinically relevant genes spanning 115 Kb of genome size (whole coding sequence) that covers all major mutations like SNV and InDels linked to breast cancer.

ctDNA Breast gene list

AKT1

APC

AR

BRCA1

BRCA2

CCND1

CDH1

EGFR

ERBB2

ESR1

FBXW7

FGFR1

FGFR2

GATA3

IGF1R

KIT

KRAS

MAP2K4

MAP3K1

MDM2

MYC

NF1

PIK3CA

PIK3R1

PTEN

RB1

SF3B1

TOP2A

TP53

Fusion Genes

AKAP12

CDK13

ESR1

LMNA

NKAIN2

NTRK1

TPM3

TPR

ctDNA Lung Cancer Panel

A hybridization based solution for screening 43 clinically relevant genes (coding regions of the genome) for diseases associated with genetic mutations. It covers all major mutations like SNV, and InDels, adding up to a target size of 110Kb with a hybridization-based target capture technique.

ctDNA Lung Gene list

AKT1

ALK

ARAF

ARID1A

BRAF

CBL

CDKN2A

CTNNB1

EGFR

ERBB2

HRAS

KEAP1

KMT2D

KRAS

MAP2K1

MET

MTOR

NF1

NRAS

NTRK1

NTRK2

PIK3CA

PTEN

RB1

RET

RIT1

ROS1

SETD2

SOX2

STK11

TP53

U2AF1

Fusion Genes

ALK

CCDC6

CD74

EML4

EZR

IRF2B2P

KIF5B

NTRK1

RABGAP1L

RET

ROS1

SCD4

SLC34A2

SQSTM1

TP53

TPM3

ctDNA Colorectal Cancer Panel

A hybridization based solution for targeted sequencing employing NGS. With a fast turnaround time this product provides detection and identification of 35 clinically relevant genes spanning 75 Kb of genome size (whole coding sequence) that covers all major mutations like SNV & InDels linked to colorectal cancer

ctDNA Colorectal Panel

APC

ASXL1

BRAF

CHEK2

CTNNB1

DNMT3A

EGFR

ERBB2

ERBB3

FBXW7

FGFR1

GNAS

HRAS

IDH1

IRS1

KRAS

MAP2K1

MET

NRAS

PDGFRB

PIK3CA

PTEN

SMAD4

TET2

TP53

Fusion Genes

ALK

C2orf44

CSNK1G1

ETV6

LMNA

NTRK1

NTRK3

RUNX1

TPM3

TPR

Panel Specifications

Panel

ctDNA Lung

ctDNA Breast

ctDNA Colorectal

No. of genes covered

48

37

35

Target size

75

115

110

Mutations covered

SNV, InDels, CNV

SNV, InDels, CNV

SNV, InDels

Workflow time

Library Prep. (∼4 hrs.) + Hyb. (∼8 hrs.)

Pack size

24/96 samples

Sample type

Blood/ AF/ Tissue/ CVS

Sample input

50-500 ng

Covered region

Whole CDS, Mitochondrial genome, Hotspots

Shelf life

12 months (in recommended storage conditions)

Platform compatibility

Illumina, Thermo Fisher (Ion Torrent), MGI, Element Biosciences

Automation option

G2M EZY-Auto Prep

Data analysis platform

G2M CliSeq Interpreter.

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