Oncological investigation in a cancer patient plays a significant role in order to develop an effective treatment plan. Comprehensive information related to tumors is required when associated genes, mutations and variants are investigated. Solid tumor biopsy though effective, has its own challenges. Tumor sample collection is a painful and costly process making this investigation difficult. Liquid biopsy is a method where circulating tumor DNA (ctDNA) shed from the tumor is extracted and sequenced. This unveils the necessary information regarding cancer causing mutations in the related genes enhancing clinicians to decide the best course of treatment.
G2M offers a panel for liquid biopsy covering major cancer types like Lung, Breast and Colorectal cancer. This panel gives a detailed insight on cancer related genes to uncover known as well as novel variants for further investigation
A hybridization based solution for targeted sequencing employing NGS. With a fast turnaround time this product provides detection and identification of 36 clinically relevant genes spanning 115 Kb of genome size (whole coding sequence) that covers all major mutations like SNV and InDels linked to breast cancer.
ctDNA Breast gene list |
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AKT1 |
APC |
AR |
BRCA1 |
BRCA2 |
CCND1 |
CDH1 |
EGFR |
ERBB2 |
ESR1 |
FBXW7 |
FGFR1 |
FGFR2 |
GATA3 |
IGF1R |
KIT |
KRAS |
MAP2K4 |
MAP3K1 |
MDM2 |
MYC |
NF1 |
PIK3CA |
PIK3R1 |
PTEN |
RB1 |
SF3B1 |
TOP2A |
TP53 |
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Fusion Genes |
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AKAP12 |
CDK13 |
ESR1 |
LMNA |
NKAIN2 |
NTRK1 |
TPM3 |
TPR |
A hybridization based solution for screening 43 clinically relevant genes (coding regions of the genome) for diseases associated with genetic mutations. It covers all major mutations like SNV, and InDels, adding up to a target size of 110Kb with a hybridization-based target capture technique.
ctDNA Lung Gene list |
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AKT1 |
ALK |
ARAF |
ARID1A |
BRAF |
CBL |
CDKN2A |
CTNNB1 |
EGFR |
ERBB2 |
HRAS |
KEAP1 |
KMT2D |
KRAS |
MAP2K1 |
MET |
MTOR |
NF1 |
NRAS |
NTRK1 |
NTRK2 |
PIK3CA |
PTEN |
RB1 |
RET |
RIT1 |
ROS1 |
SETD2 |
SOX2 |
STK11 |
TP53 |
U2AF1 |
Fusion Genes |
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ALK |
CCDC6 |
CD74 |
EML4 |
EZR |
IRF2B2P |
KIF5B |
NTRK1 |
RABGAP1L |
RET |
ROS1 |
SCD4 |
SLC34A2 |
SQSTM1 |
TP53 |
TPM3 |
A hybridization based solution for targeted sequencing employing NGS. With a fast turnaround time this product provides detection and identification of 35 clinically relevant genes spanning 75 Kb of genome size (whole coding sequence) that covers all major mutations like SNV & InDels linked to colorectal cancer
ctDNA Colorectal Panel |
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APC |
ASXL1 |
BRAF |
CHEK2 |
CTNNB1 |
DNMT3A |
EGFR |
ERBB2 |
ERBB3 |
FBXW7 |
FGFR1 |
GNAS |
HRAS |
IDH1 |
IRS1 |
KRAS |
MAP2K1 |
MET |
NRAS |
PDGFRB |
PIK3CA |
PTEN |
SMAD4 |
TET2 |
TP53 |
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Fusion Genes |
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ALK |
C2orf44 |
CSNK1G1 |
ETV6 |
LMNA |
NTRK1 |
NTRK3 |
RUNX1 |
TPM3 |
TPR |
Panel |
ctDNA Lung |
ctDNA Breast |
ctDNA Colorectal |
No. of genes covered |
48 |
37 |
35 |
Target size |
75 |
115 |
110 |
Mutations covered |
SNV, InDels, CNV |
SNV, InDels, CNV |
SNV, InDels |
Workflow time |
Library Prep. (∼4 hrs.) + Hyb. (∼8 hrs.) |
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Pack size |
24/96 samples |
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Sample type |
Blood/ AF/ Tissue/ CVS |
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Sample input |
50-500 ng |
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Covered region |
Whole CDS, Mitochondrial genome, Hotspots |
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Shelf life |
12 months (in recommended storage conditions) |
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Platform compatibility |
Illumina, Thermo Fisher (Ion Torrent), MGI, Element Biosciences |
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Automation option |
G2M EZY-Auto Prep |
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Data analysis platform |
G2M CliSeq Interpreter. |