Whole Exome Sequencing has now been accepted as a significant approach for unveiling known as well as novel variants in a variety of diseases. Clinical studies have been boosted by the whole exome approach since it emerges as a cost effective alternative for whole genome sequencing. Focusing only on the coding region of the genome, which contains more than 80% of the disease causing genes, the data possess a huge potential in identifying mutations in the exonic region making it beneficial for personalizing patient treatment.
G2M Whole Exome Sequencing (WES) panel is designed using a hybridization-capture based approach for targeting complete exonic regions and identifying major germline mutations. The kit has been optimized to exhibit Q30+ read accuracy and target around 21500 significant genes based on databases like RefSeq, CCDS and GENCODE.
Hands on time required |
8 hours |
Multiplexing |
24/96 samples |
Sample type |
Blood/ AF/ Tissue/ CVS |
Sample input |
50-500 ng |
Covered Region |
Whole CDS, Mitochondrial genome, Hotspots |
No. of genes covered |
∼21500 |
Target size |
3.8 Mb |
Shelf life |
12 months (in recommended storage conditions) |
Automation option |
G2M EZY-Prep |
Data Analysis tool |
G2M CliSeq Interpreter. |
G2M offers flexibility of choosing a sequencing platform suitable for user’s requirement while keeping the gene content constant.
Illumina |
iSeq, MiniSeq, MiSeq, NextSeq550, NextSeq 1000/2000, NovaSeq 6000, NovaSeq X series. |
Thermo Fisher (Ion Torrent) |
S5, S5 plus systems |
MGI |
T series, G series, E series |
Element Biosciences |
Avity |
The panel adapts a simple, straight-forward workflow enhancing a quick turnaround time . The workflow is divided into.
A : Library Prepration :This step includes end repair, adapter ligation, purification using MPB clean up beads and index ligated.
B : Hybridization and target enrichment :This step includes hybridization- capture of target region using biotinylated probes and selection of target region using MSB beads. It is followed by post capture amplification, purification and QC.
