Cancer genomics has been proven to be a major breakthrough to understand significant mutations in relevant genes. A comprehensive cancer profile which includes mutations like SNV, CNV and InDels as well as prognostic biomarkers like Microsatellite Instability (MSI) and Tumor Mutation Burden (TMB) boosts the efficacy of precision oncology.
Genes 2Me offers a panel detecting all variant types and immuno-oncology biomarkers such as MSI and TMB for an all-inclusive information on the patient’s genomic profile. The panel is also designed to detect Epstein-Barr Virus (EBV) and Human Papilloma Virus (HPV) for a better overview. Panel identifies around 681 genes covering the whole coding sequences (CDS), hotspots and fusion genes with a target size of around 1.7 Mb.
Workflow time |
Library Prep. (∼4 hrs.) + Hyb. (∼8 hrs.) |
Pack size |
24/96 reactions |
Sample type |
FFPE, Fresh frozen tissue |
Sample input |
50-500 ng |
Covered region |
Whole CDS, Hotspots, Fusion genesm |
No. of genes covered |
∼681 |
Biomarkers covered |
TMB, MSI, HRR gene |
Target size |
1.7 Mb |
Shelf life |
12 months (in recommended storage conditions) |
Platform compatibility |
Illumina, Thermo Fisher (Ion Torrent), MGI, Element Biosciences |
Automation option |
G2M EZY-Auto Prep |
Data analysis platform |
G2M CliSeq Interpreter. |
The panel adapts a simple, straight-forward workflow enhancing a quick turnaround time. The workflow is divided into:
A : Library Prepration :DNA/ RNA extracted from the samples are converted into libraries which are ready for hybridization. This step includes end repair, adapter ligation, purification using MPB clean up beads and index ligated.
B : Hybridization and target enrichment :This step includes hybridization- capture of target region using biotinylated probes and selection of target region using MSB beads. It is followed by post capture amplification, purification and QC.
