The SpiNXT Bacterial RNA Extraction kit offers a simple, efficient & structured system for purification of total RNA from Gram-positive and Gram-negative bacterial cultures. The kit makes use of silica membrane based spin column technology using a convenient spin column format eliminating the need of alcohol precipitation or toxic phenol/chloroform extractions and is thus ideal for safe and convenient extraction.
RNA Mini Columns | Collection Tubes | Buffer RLB | Buffer RLB | Buffer RLB-1 | Buffer RLB-5 | Buffer W1 | Buffer W2 | Proteinase K | Protease Dissolve Buffer | Lysozyme | Buffer EB
Recommended Input Amount : ~1.2ml of bacterial culture
Binding Mechanism : Silica membrane spin column technology
Binding Capacity : 15-20µg
Elution Volume : ≥30µl
Purity : A260/280 - 2±0.1, A260/230 - 2.0±0.1
Expected Yield : ≥5μg ( depending upon the type, quality & quantity of the starting material used)
Reverse Transcriptase PCR (RT-PCR)
Northern Blotting
cDNA Library construction
RNA based analysis
Reverse Transcriptase PCR (RT-PCR)
Format |
Mini spin column |
Technology |
Silica membrane |
Sample Type |
1.2ml of an overnight bacterial broth culture |
Yield |
≥5μg |
Target |
Total RNA |
A260/280 |
2.0 ± 0.1 |
Available in pack sizes |
50 Rxn, 250 Rxn |
Throughput Compatibility |
Manual |
CAT # |
DESCRIPTION |
UNITS |
G2M121220 |
SpiNXT Bacterial RNA Extraction kit |
50 Rxn |
G2M121320 |
SpiNXT Bacterial RNA Extraction kit |
250 Rxn |
