MDR-Q Real-Time PCR Kit

Product Overview

Antimicrobial drug resistance (AMR) refers to the ability of microorganisms, including bacteria, viruses, fungi, and parasites, to withstand the effects of medications designed to kill or inhibit their growth. This phenomenon poses a significant global health threat, as it undermines the effectiveness of antibiotics, antivirals, anti-fungal, and anti-parasitic drugs, making infections harder to treat and increasing the risk of severe illness, complications, and death. Effective treatment of infection depends on accurate identification of the pathogen and the correct choice of antibiotics, making accurate antimicrobial susceptibility (AST) profiling of a pathogen crucial for the treatment of underlying infection.

To address this critical clinical need, Genes2Me introduces the MDR-Q Real-Time PCR Kit (Antimicrobial Resistance), a comprehensive molecular diagnostic solution for the qualitative detection of eighteen antimicrobial resistance genes in human clinical samples. The kit includes a comprehensive set of reagents and protocols for the accurate and efficient detection and differentiation of resistance genes encoding resistance to Carbapenems, Vancomycin, Methicillin, Colistin, Extended spectrum beta lactams (ESBLs), AmpC beta lactamase, Trimethoprim/Sulfamethoxazole (SXT), and plasmid mediated quinolone resistance (PMQR). The assay is intended to be performed by a laboratory professional in a clinical laboratory.

Targets:
18 Antimicrobial Resistance (AMR) Genes
Technology:
Real-Time PCR (Hydrolysis Probe Chemistry)
Detection:
Qualitative (Detected / Not Detected)
Sample Type:
Human Whole Blood
Certification:
IVD
MDR-Q Comprehensive

How the Assay Works

The MDR-Q Kit is based on real time PCR technology using specific oligonucleotide primers and dual labeled fluorescent hydrolysis probes that target conserved regions of the resistance genes. Each probe is labeled with a reporter dye at the 5’ end and a quencher at the 3’ end. During PCR amplification, the probes hybridize to their complementary DNA targets, and as Taq polymerase extends the primers, it cleaves the bound probe through its exonuclease activity, separating the reporter from the quencher and producing fluorescence proportional to the amount of target DNA present. The assay is conducted across five separate reaction tubes. Tube 1 targets the carbapenem resistance genes VIM (FAM), OXA-48 (HEX), IMP (Texas Red), and NDM (Cy5).

Tube 2 targets the Methicillin resistance gene mecA (FAM), CTX-M (HEX), vanA (Texas Red), and the Colistin resistance gene MCR-1 (Cy5). Tube 3 targets TEM (FAM), vanB (HEX), SHV (Texas Red), and the AmpC beta lactamase resistance gene citM (Cy5). Tube 4 targets the Trimethoprim/Sulfamethoxazole resistance genes dfrA1 (FAM) and dfrA5 (HEX), sul2 (Texas Red), and the plasmid mediated quinolone resistance gene aac6’-Ib-cr (Cy5). Tube 5 targets the carbapenem resistance genes KPC (FAM) and OXA-23 (HEX), together with an Internal Control (Cy5).

Clinical Relevance

Antimicrobial resistance undermines the effectiveness of antibiotics, antivirals, anti-fungal, and anti-parasitic drugs, making infections harder to treat and increasing the risk of severe illness, complications, and death. Rapid molecular detection of resistance genes conferring resistance to Carbapenems, Vancomycin, Methicillin, Colistin, Extended spectrum beta lactams, AmpC beta lactamase, Trimethoprim/Sulfamethoxazole, and plasmid mediated quinolone resistance supports accurate antimicrobial susceptibility profiling, helping clinicians choose effective antibiotic therapy and supporting antimicrobial stewardship in the underlying infection.

Sample Type & Reporting Output

Sample Type:
Human Whole Blood
Reporting Output:
Qualitative detection of antimicrobial resistance genes (Detected / Not Detected)
Targets Detected:
18 AMR genes and Internal Control across 5 primer probe tubes

Key Features & Benefits

Comprehensive AMR Gene Panel

Qualitative detection of eighteen antimicrobial resistance genes spanning Carbapenems, Vancomycin, Methicillin, Colistin, Extended spectrum beta lactams (ESBLs), AmpC beta lactamase, Trimethoprim/Sulfamethoxazole (SXT), and plasmid mediated quinolone resistance (PMQR), across 5’ probe tubes.

Hydrolysis Probe Chemistry

Dual-labelled fluorescent hydrolysis probes targeting conserved regions of resistance genes deliver specific, real time fluorescence detection proportional to target DNA present.

Integrated Internal Control

An Internal Control included in Tube 5 validates assay performance in every run, supporting confidence in result interpretation.

High Analytical Performance

100% analytical sensitivity and 100% analytical specificity, with a limit of detection of 100 copies per ml and a measuring range of 10 to 109 copies per reaction.

Flexible Compatibility

Validated on leading Real-Time PCR platforms including Thermo Fisher QuantStudio 5, Bio-Rad CFX96, Roche LightCycler 480, and Genes2Me RapiCycler 96

Long Shelf Life

he kit has a shelf life of 12 months from the date of manufacture and remains stable until the labelled expiration date when stored as specified.

Applications

  • Rapid molecular screening for antimicrobial resistance genes to guide selection of effective antibiotic therapy in clinical samples
  • Supporting antimicrobial stewardship programs by identifying resistance mechanisms ahead of or alongside conventional culture-based susceptibility testing
  • Infection control screening and surveillance for multidrug resistant organisms in hospital and critical care settings
  • Epidemiological monitoring of Carbapenem, Vancomycin, Methicillin, Colistin, ESBL, AmpC, SXT, and PMQR resistance gene prevalence
  • Research applications studying the molecular basis and spread of antimicrobial resistance genes

Analytical Performance

  • Analytical sensitivity of 100% and analytical specificity of 100% for detection of antimicrobial resistance genes
  • Trueness (bias) of 98% and accuracy (resulting from trueness and precision) of 99%
  • Precision with a coefficient of variation of less than 3% for both repeatability and reproducibility
  • Limit of detection of 100 copies per ml, with a measuring range of 10 to 109 copies per reaction and linearity of R squared equals 0.998
  • Ct value cut off less than or equal to 36 for differentiating positive and negative results
  • Well controlled identification and control of known relevant endogenous and exogenous interfering substances, including human genomic DNA, NaCl, BSA, and whole blood
  • No cross reactivity observed against Mycobacterium tuberculosis, Haemophilus influenzae, and methicillin susceptible Staphylococcus
  • Compatible with QuantStudio 5, CFX96, LightCycler 480, and RapiCycler 96 platforms
  • Manufactured under strict quality standards with reagent stability maintained under recommended storage conditions of negative 30 degrees Celsius to negative 10 degrees Celsius

With the MDR-Q Real-Time PCR Kit (Antimicrobial Resistance), laboratories can achieve sensitive, specific, and clinically meaningful detection of the genes responsible for multidrug resistance. By supporting accurate antimicrobial susceptibility profiling across a comprehensive AMR gene panel, the kit helps clinicians make timely, informed decisions for patient care and antimicrobial stewardship. Empower your laboratory with the MDR-Q Real-Time PCR Kit, because rapid molecular insights can make a lasting difference in patient outcomes.

Data Analysis and Report

MDR-Q Comprehensive

MDR-Q Real-Time PCR Kit (Antimicrobial Resistance) specific amplification plot of Treponema pallidum Positive Control (HEX Channel) along with Internal Control (Cy5 Channel)

Ordering Information

Commercial Name Old Cat No. New Cat No. Pack Size
MDR-Q Real-Time PCR Kit (Antimicrobial Resistance) G2M707521 G610016 50 Tests

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Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.

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